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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

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Related Experiment Video

Updated: Jan 20, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
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A sugarcane mosaic virus vector for gene expression in maize.

Yu Mei1, Guanjun Liu2, Chunquan Zhang3

  • 1Department of Plant Pathology and Microbiology Iowa State University Ames Iowa.

Plant Direct
|August 15, 2019
PubMed
Summary

Researchers developed a new sugarcane mosaic virus (SCMV) vector for protein expression in maize. This SCMV vector efficiently expresses foreign genes, aiding maize gene function studies.

Keywords:
gene expressiongreen fluorescent proteinmaizemonocotpotyvirusvector

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Area of Science:

  • Plant Virology
  • Molecular Biology
  • Genetics

Background:

  • Maize (Zea mays) is a vital crop and model organism for plant science.
  • Virus-based vectors offer transient gene expression and silencing for studying gene function.
  • Developing versatile tools is crucial for advancing maize research.

Purpose of the Study:

  • To engineer a sugarcane mosaic virus (SCMV) vector for heterologous protein expression in maize.
  • To assess the SCMV vector's capacity for foreign gene insertion and expression.
  • To evaluate the stability and applicability of the SCMV expression system in diverse maize genotypes.

Main Methods:

  • Construction of an infectious SCMV cDNA clone with foreign gene insertions.
  • Introduction of recombinant SCMV expressing GFP, GUS, or BAR into sweet corn plants.
  • Analysis of SCMV genome tolerance for inserted sequences and coding sequence stability.

Main Results:

  • The SCMV genome accommodates insertions up to 1,809 nucleotides at the P1/HC-Pro junction.
  • Recombinant SCMV successfully expressed foreign proteins (GFP, GUS, BAR) in maize.
  • GFP and BAR coding sequences showed greater stability than the larger GUS sequence.

Conclusions:

  • A novel SCMV-based expression vector was successfully developed for maize.
  • This SCMV vector facilitates the study of gene function in maize by enabling transient protein expression.
  • The vector's broad infectivity across maize lines suggests its utility for diverse genetic studies.