Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nanometer-scale measurements using video light microscopy.

B J Schnapp1, J Gelles, M P Sheetz

  • 1Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis.

Cell Motility and the Cytoskeleton
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.

Nature communications·2019
Same author

Cell spreading as a hydrodynamic process.

Soft matter·2013
Same author

Fabrication of elastomer Pillar Arrays with Modulated Stiffness for Cellular Force Measurements.

Journal of vacuum science & technology. B, Microelectronics and nanometer structures : processing, measurement, and phenomena : an official journal of the American Vacuum Society·2011
Same author

Gold-Tipped Elastomeric Pillars for Cellular Mechanotransduction.

Journal of vacuum science & technology. B, Microelectronics and nanometer structures : processing, measurement, and phenomena : an official journal of the American Vacuum Society·2010
Same author

Fabrication of Nanoscale Bioarrays for the Study of Cytoskeletal Protein Binding Interactions Using Nanoimprint Lithography.

Journal of vacuum science & technology. B, Microelectronics and nanometer structures : processing, measurement, and phenomena : an official journal of the American Vacuum Society·2009
Same author

Cell traction.

Current protocols in cell biology·2008
Same journal

Mechanics and dynamics of the cytoskeleton. Proceedings of a FEBS workshop. June 22-26, 2008. Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/ FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture.

Cell motility and the cytoskeleton·2009
Same journal

Centriole symmetry: a big tale from small organisms.

Cell motility and the cytoskeleton·2009
Same journal

Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration.

Cell motility and the cytoskeleton·2009
See all related articles

Quantitative analysis of diffraction images enables nanometer-level tracking of cellular structures. This advanced microscopy technique achieves high precision for observing molecular motor protein motion.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Traditional light microscopy has resolution limits (e.g., Raleigh limit of 0.2 micron).
  • Advancements in video and digital image processing enhance contrast in microscopic images.
  • Observing structures smaller than the diffraction limit is crucial for understanding cellular dynamics.

Purpose of the Study:

  • To demonstrate quantitative analysis of diffraction images for nanometer-level information extraction.
  • To apply this method for precise tracking of molecular motors.
  • To achieve sub-wavelength resolution for observing cellular components.

Main Methods:

  • Utilizing video and digital image processing to amplify contrast in light microscopic images.
  • Analyzing diffraction images of cellular structures.

Related Experiment Videos

  • Developing a method for real-time tracking of kinesin-coated beads on microtubules.

    • Main Results:

    • Achieved real-time observation of diffraction images of structures 10 times smaller than the Raleigh limit.
    • Demonstrated the extraction of motion and structure information at the nanometer level.
    • Tracked kinesin-coated bead motion on microtubules with 1-2 nm precision.

    Conclusions:

    • Quantitative analysis of diffraction images is a powerful tool for nanometer-level investigations.
    • This technique significantly surpasses traditional resolution limits in light microscopy.
    • Enables precise study of molecular mechanisms, such as motor protein movement.