Related Experiment Video
Updated: Jan 20, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Optical coherence tomography-guided dynamic focusing for combined optical and mechanical scanning multimodal
Arash Dadkhah1, Shuliang Jiao1
1Florida International University, Department of Biomedical Engineering, Miami, Florida, United States.
Abstract:
To achieve fast imaging and large field of view (FOV), we improved our multimodal imaging system, which integrated optical resolution photoacoustic microscopy, optical coherence tomography (OCT), and confocal fluorescence microscopy in one platform, by combining optical scanning with mechanical scanning. To ensure good focusing of the objective lens over all the imaged area, we employed OCT-guided dynamic focusing. Different from our previous point-by-point dynamic focusing, we employed an area-by-area focusing adjustment strategy, in which each fast optical scanning area has a fixed focusing depth. We have demonstrated the performance of the system by imaging biological samples ex vivo (plant leaf) and in vivo (mouse ear). The system has achieved uniform resolution in an FOV of 10 mm × 10 mm with an imaging time of about 5 min.
More Related Videos
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Properties of Enantiomers and Optical Activity
Leaky Scanning
Reaction Mechanisms
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
Focusing of Light in the Eye

