Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interference of Staphylococcus aureus lipase with human granulocyte function.

J Rollof1, J H Braconier, C Söderström

  • 1Department of Infectious Diseases, University Hospital, Lund, Sweden.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|August 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurements of the natural background of radioxenon isotopes in soil gas.

Journal of environmental radioactivity·2025
Same author

Measurement of radioxenon and radioargon in soil gas collected in the region of Kvarntorp, Sweden.

Journal of environmental radioactivity·2020
Same author

Airborne concentrations and chemical considerations of radioactive ruthenium from an undeclared major nuclear release in 2017.

Proceedings of the National Academy of Sciences of the United States of America·2019
Same author

Measurements of <sup>106</sup>Ru in Sweden during the autumn 2017: Gamma-ray spectrometric measurements of air filters, precipitation and soil samples, and in situ gamma-ray spectrometry measurement.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2018
Same author

Association between serum interleukin-17A and clinical response to tofacitinib and etanercept in moderate to severe psoriasis.

Clinical and experimental dermatology·2018
Same author

Evaluation of an early warning system for airborne radionuclides.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2016

Staphylococcus aureus lipase impacts granulocyte function, impairing directed movement and bacterial killing. These effects, partly independent of enzymatic activity, suggest lipase is a key virulence factor.

Area of Science:

  • Immunology
  • Microbiology
  • Biochemistry

Background:

  • Staphylococcus aureus is a common pathogen.
  • Granulocytes are key immune cells fighting bacterial infections.
  • Lipases are enzymes that hydrolyze lipids.

Purpose of the Study:

  • To investigate the influence of Staphylococcus aureus lipase on granulocyte function and morphology.
  • To determine if lipase activity is solely responsible for its effects on granulocytes.

Main Methods:

  • Purified Staphylococcus aureus lipase was used.
  • Granulocyte chemotaxis was assayed using an agarose system.
  • Phagocytic killing of Staphylococcus aureus by granulocytes was measured.
  • Granulocyte morphology and surface structure were examined.

Related Experiment Videos

  • Heat inactivation of lipase was performed to assess the role of enzymatic activity.

    • Main Results:

    • Lipase exhibited chemotactic properties and enhanced granulocyte movement at low concentrations.
    • Higher lipase concentrations progressively reduced granulocyte chemotaxis and phagocytic activity in a dose-dependent manner.
    • Lipase altered granulocyte surface structure, causing denudation and loss of microvilli.
    • Effects were partially retained after heat inactivation, indicating non-enzymatic contributions.

    Conclusions:

    • Staphylococcus aureus lipase significantly impairs granulocyte function, affecting both movement and killing capabilities.
    • The observed effects are partly independent of lipase's enzymatic activity, suggesting other mechanisms are involved.
    • Staphylococcal lipase may act as a crucial virulence factor, contributing to deep-site infections.