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Related Experiment Videos

A new bioassay for terbinafine.

M Häuser1, H J Schmitt, E M Bernard

  • 1Infectious Diseases Service, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|August 1, 1988
PubMed
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A novel agar diffusion bioassay accurately measures the antifungal drug terbinafine in serum. This sensitive method uses Aspergillus flavus to detect terbinafine concentrations as low as 0.2 microgram/ml.

Area of Science:

  • Mycology
  • Analytical Chemistry
  • Pharmacology

Background:

  • Terbinafine is a widely used antifungal medication.
  • Accurate measurement of drug levels in biological fluids is crucial for therapeutic drug monitoring.
  • Existing methods for terbinafine quantification may lack simplicity or sensitivity.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and specific bioassay for terbinafine in human serum.
  • To establish the quantitative range of terbinafine detectable by the assay.

Main Methods:

  • An agar diffusion bioassay was optimized using a specific strain of Aspergillus flavus as the test microorganism.
  • Terbinafine standards were prepared in pooled human serum at known concentrations.
  • Agar plates were inoculated with Aspergillus flavus spores, and serum samples containing terbinafine were applied to wells.

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  • Inhibition zones were measured, and a standard curve was generated to correlate zone diameter with terbinafine concentration.

    • Main Results:

    • The bioassay demonstrated sensitivity in detecting terbinafine concentrations ranging from 0.2 to 6.4 micrograms/ml in serum.
    • The method was found to be specific for terbinafine, with minimal interference from other serum components.
    • Reproducible inhibition zone diameters were observed for known terbinafine concentrations.

    Conclusions:

    • A simple, sensitive, and specific agar diffusion bioassay for the quantification of terbinafine in serum has been successfully developed.
    • This assay provides a reliable method for measuring therapeutic terbinafine levels, aiding in clinical management.
    • The assay's simplicity and sensitivity make it suitable for routine laboratory use.