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miR-5195-3p Suppresses Cell Proliferation and Induces Apoptosis by Directly Targeting NEDD9 in Osteosarcoma

Luowen Wang1, Genbing Shi1, Donghui Zhu1

  • 1Department of orthopedics, Shanghai Electric Power Hospital, Shanghai, China.

Cancer Biotherapy & Radiopharmaceuticals
|August 15, 2019
PubMed

Insights

MicroRNAs (miRs) like miR-5195-3p are crucial in osteosarcoma (OS) development. This study shows miR-5195-3p inhibits OS cell growth and promotes apoptosis by targeting NEDD9, suggesting a new therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miR) expression is linked to osteosarcoma (OS) development and progression.
  • Understanding the specific roles of miRs in OS is critical for identifying novel therapeutic targets.

Purpose of the Study:

  • To investigate the biological function of miR-5195-3p in osteosarcoma.
  • To elucidate the molecular mechanisms underlying miR-5195-3p's role in OS.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to assess miR-5195-3p expression.
  • Transfection of OS cell lines (MG-63, U2OS) with miR-5195-3p mimics.
  • Functional assays including CCK-8, colony formation, flow cytometry, and Hoechst staining to evaluate cell proliferation and apoptosis.
  • Analysis of neural precursor cell expressed developmentally downregulated protein 9 (NEDD9) expression.

Main Results:

  • miR-5195-3p was found to be downregulated in OS tissues and cell lines.
  • Overexpression of miR-5195-3p significantly reduced OS cell proliferation and induced apoptosis.
  • NEDD9 expression was inversely correlated with miR-5195-3p levels.
  • Ectopic expression of NEDD9 reversed the anti-proliferative and pro-apoptotic effects of miR-5195-3p.

Conclusions:

  • The miR-5195-3p/NEDD9 axis plays a significant role in regulating osteosarcoma cell behavior.
  • miR-5195-3p acts as a tumor suppressor in osteosarcoma.
  • The miR-5195-3p/NEDD9 pathway represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
NEDD9apoptosiscell proliferationmiR-5195-3posteosarcoma

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