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Updated: Jan 20, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
Immortalization of a cell line with neural stem cell characteristics derived from mouse embryo brain
Cristina Almengló1, Tamara González-Mosquera1, Pilar Caamaño2
1Departamento de Fisioloxia, Facultade de Medicina and Centro de Investigación en Medicina Molecular y Enfermedades Crónicas (CiMUS). Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
Background:
Neural stem cells (NSC) have been extensively used as a tool to investigate the mechanisms responsible for neural repair, and they have been also considered as the source for a series of promising replacement therapies in various neurodegenerative diseases. However, their use is limited by their relative rarity and anatomical localization, and also because, the methods for isolation and characterization are usually time consuming and have some technical limitations.
Results:
In this study, we describe a resource and method for obtaining immortalized cells with NSC characteristics obtained from mouse brain embryo.
Conclusions:
Because these cells can be maintained indefinitely in culture, they may constitute a permanent source of NSC that can be used for research studies on neural development and regeneration.
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