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MicroRNA expression in infertile men: its alterations and effects

Maryam Kiani1, Mohammad Salehi2,3, Asghar Mogheiseh1

  • 1Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, shiraz, Iran.

Zygote (Cambridge, England)
|August 16, 2019
PubMed

Insights

Analyzing microRNAs (miRNAs) in male infertility offers a non-invasive diagnostic approach. Altered miRNA expression in semen, sperm, and testicular samples can identify causes of unexplained male infertility.

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Biomarker Discovery

Background:

  • Male infertility affects over half of infertility cases, with genetic factors playing a crucial role.
  • Semen analysis alone is insufficient for diagnosing idiopathic male infertility.
  • MicroRNAs (miRNAs) are emerging as non-invasive biomarkers for reproductive health.

Purpose of the Study:

  • To review recent research on altered miRNA expression in infertile males.
  • To explore the diagnostic potential of miRNAs in various types of unexplained male infertility.
  • To highlight the necessity of non-invasive diagnostic methods for male infertility.

Main Methods:

  • Literature review of studies investigating miRNA expression in semen, sperm, and testicular biopsies.
  • Analysis of microarray data for miRNA expression profiling.
  • Confirmation of miRNA levels using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).

Main Results:

  • Significant alterations in miRNA expression patterns are observed in infertile males.
  • Specific miRNAs show differential expression correlating with spermatogenic and histopathologic injury.
  • miRNA profiling provides valuable molecular insights beyond traditional semen analysis.

Conclusions:

  • Altered miRNA expression serves as a promising non-invasive diagnostic biomarker for male infertility.
  • miRNA analysis can aid in identifying the causes of unexplained male infertility.
  • Developing non-invasive miRNA-based diagnostics is crucial for improving male reproductive health assessments.
Keywords:
BiomarkerMaleMicroRNANon-invasiveUnexplained infertility

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