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[Overexpression of the long non-coding RNA ADAMTS9-AS2 suppresses colorectal cancer proliferation and metastasis]

Xiaoyun Bu1, Ang Qin2, Zhi Luo1

  • 1Department of Colorectal Surgery, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China.

Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences
|August 16, 2019
PubMed
Abstract

Insights

The long non-coding RNA ADAMTS9-AS2 is down-regulated in colorectal cancer (CRC). Higher ADAMTS9-AS2 expression predicts better relapse-free survival and inhibits CRC cell proliferation and metastasis, suggesting a tumor suppressor role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) remains a significant global health challenge.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of ADAMTS9-AS2 in CRC is not well understood.

Purpose of the Study:

  • To investigate the expression, clinical significance, and biological function of ADAMTS9-AS2 in colorectal cancer (CRC).
  • To determine if ADAMTS9-AS2 can serve as a prognostic biomarker for CRC patients.

Main Methods:

  • Gene microarray and real-time PCR to assess ADAMTS9-AS2 expression in CRC tissues and cells.
  • Kaplan-Meier and Cox regression analyses to evaluate the prognostic value of ADAMTS9-AS2 for relapse-free survival (RFS) in early-stage CRC.
  • In vitro assays (cell counting kit-8, colony formation, Transwell) to determine the functional role of ADAMTS9-AS2 in CRC cell proliferation, migration, and invasion.

Main Results:

  • ADAMTS9-AS2 expression was significantly down-regulated in CRC tissues and cells compared to adjacent non-tumor tissues.
  • High ADAMTS9-AS2 expression was correlated with a better 5-year RFS rate (83.8% vs 73.5%, P=0.041).
  • ADAMTS9-AS2 was identified as an independent prognostic factor for RFS in early-stage CRC (HR=0.528, P=0.028).
  • Overexpression of ADAMTS9-AS2 inhibited CRC cell proliferation, migration, and invasion, while its suppression promoted these processes.

Conclusions:

  • ADAMTS9-AS2 is a valuable prognostic biomarker for colorectal cancer.
  • ADAMTS9-AS2 functions as a tumor suppressor in CRC by inhibiting cell proliferation and metastasis.

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