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Simultaneous quantification of lidocaine and prilocaine in human plasma by LC-MS/MS and its application in a human
Siva Sankara Rao Yadlapalli1, Naresh Kumar Katari1, Surendra Babu Manabolu Surya1
1Department of Chemistry, GITAM University, Hyderabad, 502329, India.
Objective:
The aim of the work was to develop and validate a simple, sensitive and selective Liquid chromatography with Mass spectroscopic method for simultaneous quantification of lidocaine and prilocaine in human plasma.
Design And Methods:
Analytes and the internal standards from human plasma were extracted by using solid- phase extraction technique using Waters Oasis® HLB 1 cc (30 mg) cartridges. The reconstituted samples were chromatographed on Phenomenex Kinetex EVO 4.6*100 mm 2.6 μ 100A column by using a mixture of acetonitrile and 5 mM ammonium acetate buffer (80:20, v/v) as the mobile phase at a flow rate of 0.6 mL/min.
Results:
The method was validated over the concentration range of 0.10-201.80 ng/mL for lidocaine and 0.10-201.66 ng/mL for prilocaine. The calibration curve obtained was linear.
Conclusion:
Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 3.0 min for each sample, make it possible to analyze more than 350 human plasma samples per day. The proposed method was found applicable for pharmacokinetic studies.
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