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Updated: Jan 20, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
A promising detection candidate for flagellated Salmonella spp
Yi Yang1,2,3, Jiangying Zhang1,2,3, Chunhong Zhu4
1College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Researchers developed a new rapid detection method for flagellated Salmonella using a monoclonal antibody (MAb 3E2) targeting Salmonella flagellin. This MAb-based Slide Agglutination Test (SAT) offers faster and specific identification of Salmonella in samples.
Area of Science:
- Immunology
- Microbiology
- Bacteriology
Background:
- Salmonella is a significant pathogen affecting both humans and animals.
- Flagellin (FliC) is a key surface protein in flagellated Salmonella strains.
- Developing specific and rapid detection methods for Salmonella is crucial.
Purpose of the Study:
- To generate monoclonal antibodies (MAbs) against a conserved region of Salmonella flagellin (FliC').
- To develop and evaluate a rapid detection assay for flagellated Salmonella using the generated MAbs.
Main Methods:
- Bioinformatics analysis identified a conserved region of FliC' for recombinant protein (rFliC') production.
- BALB/c mice were immunized with rFliC' to produce MAbs.
- Hybridoma technology generated MAbs, with MAb 3E2 selected for further testing via immunogold electron microscopy, Western blot, and Slide Agglutination Test (SAT).
Main Results:
- Monoclonal antibody 3E2 specifically recognized Salmonella flagellin and flagella.
- MAb 3E2 demonstrated direct agglutination activity against flagellated Salmonella strains.
- The MAb 3E2-based SAT showed high specificity, reacting only with flagellated Salmonella and not with non-flagellated Salmonella or other bacteria.
- Testing on 369 raw samples from laying hens confirmed MAb 3E2-SAT's ability to specifically detect Salmonella, yielding results faster than conventional culture methods.
Conclusions:
- Monoclonal antibodies against a conserved region of Salmonella flagellin were successfully prepared.
- The MAb 3E2-based Slide Agglutination Test is a promising, rapid, and specific candidate for detecting flagellated Salmonella species.
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