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A case-control study on the relationship between miRNAs single nucleotide polymorphisms and sepsis risk

Yan Zhang1, Min Li1, Lanying Bao1

  • 1Department of Emergency, Juancheng People's Hospital, Juancheng.

Medicine
|August 16, 2019
PubMed

Insights

Single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) are linked to sepsis risk. Specific miRNA SNPs, miR-187 rs12605436, miR-21 rs13137, and miR-145 rs353291, show significant associations with sepsis development and progression.

Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) are implicated in sepsis pathogenesis.
  • The role of miRNA single nucleotide polymorphisms (SNPs) in sepsis risk remains unclear.

Purpose of the Study:

  • To investigate the association between plasma miRNA levels, their SNPs, and sepsis risk.
  • To analyze the correlation between specific miRNAs and inflammatory markers in sepsis patients.

Main Methods:

  • Plasma levels of miR-187, miR-21, and miR-145 were measured in 180 sepsis patients and 180 healthy controls.
  • SNPs rs12605436 (miR-187), rs13137 (miR-21), and rs353291 (miR-145) were detected via sequencing.
  • Plasma levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were quantified using ELISA.

Main Results:

  • Sepsis patients exhibited significantly higher TNF-α and IL-6 levels compared to controls.
  • Plasma miR-187 levels were lower in sepsis patients, while miR-21 and miR-145 levels were higher.
  • miR-187 showed an inverse correlation with TNF-α and IL-6, whereas miR-21 and miR-145 showed positive correlations.
  • The T allele of miR-187 SNP rs12605436 was a risk factor for sepsis.
  • The T alleles of miR-21 SNP rs13137 and miR-145 SNP rs353291 were protective factors against sepsis.

Conclusions:

  • Plasma miRNA levels and specific miRNA SNPs are associated with sepsis occurrence.
  • These findings highlight the potential of miRNA SNPs as biomarkers for sepsis risk assessment.

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