Related Experiment Video
Updated: Jan 20, 2026
07:24
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
1.9K
A case-control study on the relationship between miRNAs single nucleotide polymorphisms and sepsis risk
Yan Zhang1, Min Li1, Lanying Bao1
1Department of Emergency, Juancheng People's Hospital, Juancheng.
Medicine
|August 16, 2019
Summary
Single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) are linked to sepsis risk. Specific miRNA SNPs, miR-187 rs12605436, miR-21 rs13137, and miR-145 rs353291, show significant associations with sepsis development and progression.
Area of Science:
- Genetics
- Molecular Biology
- Immunology
Background:
- MicroRNAs (miRNAs) are implicated in sepsis pathogenesis.
- The role of miRNA single nucleotide polymorphisms (SNPs) in sepsis risk remains unclear.
Purpose of the Study:
- To investigate the association between plasma miRNA levels, their SNPs, and sepsis risk.
- To analyze the correlation between specific miRNAs and inflammatory markers in sepsis patients.
Main Methods:
- Plasma levels of miR-187, miR-21, and miR-145 were measured in 180 sepsis patients and 180 healthy controls.
- SNPs rs12605436 (miR-187), rs13137 (miR-21), and rs353291 (miR-145) were detected via sequencing.
- Plasma levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were quantified using ELISA.
Main Results:
- Sepsis patients exhibited significantly higher TNF-α and IL-6 levels compared to controls.
- Plasma miR-187 levels were lower in sepsis patients, while miR-21 and miR-145 levels were higher.
- miR-187 showed an inverse correlation with TNF-α and IL-6, whereas miR-21 and miR-145 showed positive correlations.
- The T allele of miR-187 SNP rs12605436 was a risk factor for sepsis.
- The T alleles of miR-21 SNP rs13137 and miR-145 SNP rs353291 were protective factors against sepsis.
Conclusions:
- Plasma miRNA levels and specific miRNA SNPs are associated with sepsis occurrence.
- These findings highlight the potential of miRNA SNPs as biomarkers for sepsis risk assessment.
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
18.0K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
18.0K
01:08Nucleotide Excision Repair
40.6K
Overview
40.6K
01:38Nucleotide Excision Repair
5.0K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.0K
02:12Relationship Formation
45.2K
What do you think is the single most influential factor in determining with whom you become friends and whom you form romantic relationships? You might be surprised to learn that the answer is simple: the people with whom you have the most contact. This most important factor is proximity. You are more likely to be friends with people you have regular contact with. For example, there are decades of research that shows that you are more likely to become friends with people who live in your dorm,...
45.2K
01:23Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
412
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
412
01:28Ending Relationships
172
The dissolution of intimate relationships presents complex emotional and psychological challenges, particularly when emotional bonds are strong, the relationship is long-standing, and perceived alternatives are limited. This distress often intensifies in romantic breakups, where the initiator may experience greater turmoil than the rejected partner. Contributing factors include residual attachment, guilt over causing pain, and uncertainty about how to manage the situation. The stress is further...
172