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Related Experiment Video

Updated: Jan 20, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Acylcarnitine profiling by low-resolution LC-MS.

David Meierhofer¹

¹Mass Spectrometry Facility, Max Planck Institute for Molecular Genetics, Berlin, Germany.

|August 16, 2019

Summary

This summary is machine-generated.

A new liquid chromatography-mass spectrometry method enables the simultaneous detection of all known acylcarnitines, crucial biomarkers for metabolic disorders. This advancement aids in newborn screening and identifying novel acylcarnitine species.

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High-Resolution Respirometry in a Small-Volume Chamber

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Published on: July 25, 2025

Area of Science:

Biochemistry
Metabolomics
Analytical Chemistry

Background:

Acylcarnitines are vital for fatty acid metabolism and mitochondrial fatty acid entry.
Altered acylcarnitine levels are diagnostic for inherited metabolic disorders like fatty acid oxidation disorders and organic aciduria.
Current screening methods quantify only a subset of known acylcarnitines.

Purpose of the Study:

To develop a rapid, robust method for simultaneous detection of all known acylcarnitines.
To enhance the accuracy and scope of newborn screening for metabolic diseases.
To facilitate the identification of novel acylcarnitine species.

Main Methods:

A single liquid chromatography-mass spectrometry (LC-MS) approach was employed.
Derivatization with 3-nitrophenylhydrazine enhanced signal intensity.
A reversed-phase column provided linear elution dependent on acyl chain length, enabling elution time prediction.

Main Results:

The method successfully detected 123 acylcarnitine species using targeted low-resolution LC-MS.
Elution time was precisely predictable based on acyl chain length and chemical properties.
The method was applied to acylcarnitine profiling in mouse tissues and fluids, revealing significant compositional differences.

Conclusions:

A novel, comprehensive LC-MS method for acylcarnitine analysis has been established.
This method improves diagnostic capabilities for metabolic disorders and aids in discovering new acylcarnitines.
The technique offers a powerful tool for acylcarnitine profiling in biological samples.