Related Experiment Video
Updated: Jan 20, 2026
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
Improving Expression of Bovine Lactoferrin N-Lobe by Promoter Optimization and Codon Engineering in Bacillus subtilis
Liang Jin1, Lihong Li1, Lixian Zhou1
1Key Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology , Jiangnan University , Wuxi 214122 , P. R. China.
Abstract:
Bovine lactoferrin N-lobe plays an important key in the nonimmunological defense system. In this work, the most suitable promoter P was selected and the fragment coding bovine lactoferrin N-lobe was optimized according to codon bias of Bacillus. The recombinant plasmid pMA0911-P-mBLF-N was introduced into Baicillus subtilis 168 to create B. subtilis/pMA0911-P-mBLF-N. The bovine lactoferrin N-lobe was highly expressed at 28 °C for 15 h. Its purified protein was obtained with 16.5 mg/L and a purity of 93.6% using ammonium sulfate precipitation, Ni-NTA, and molecular exclusion. About 200 ng/mL purified bovine lactoferrin N-lobe completely inhibited cell-growth of Escherichia coli JM109 (DE3), 70.3% of Pseudomonas aeruginosa CGMCC 1.6740, and 41.5% of Staphylococcus aureus CGMCC 1.282. To our knowledge, this is the first report about active expression, purification, and characterization of bovine lactoferrin N-lobe in safe bacterium B. subtilis, which opens an available application way in the biomedical and food industries.
Related Concept Videos
02:40The Eukaryotic Promoter Region
02:40The Eukaryotic Promoter Region
01:42What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
02:07Improving Translational Accuracy
01:22Lobes of the Cerebrum
Frontal lobe
The frontal lobes, located behind the forehead, are the command center of our brain, controlling personality, intelligence, and voluntary muscle movements....
02:26tRNA Activation