Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

49.8K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
49.8K
Nucleic acids02:43

Nucleic acids

188.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
188.6K
Nucleic Acids02:43

Nucleic Acids

8.9K
8.9K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.0K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.4K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.4K
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

13.9K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pericardium decellularization in a one-day, two-step protocol.

Molecular and cellular biochemistry·2024
Same author

Development of a full-thickness acellular dermal graft from human skin: Case report of first patient rotator cuff patch augmentation repair.

Transplant immunology·2023
Same author

Last twenty-years activity of cardiovascular tissue banking in Barcelona.

Cell and tissue banking·2023
Same author

Fast protocol for the processing of split-thickness skin into decellularized human dermal matrix.

Tissue & cell·2021
Same author

Sorption and release process of polybrominated diphenyl ethers (PBDEs) from different composition microplastics in aqueous medium: Solubility parameter approach.

Environmental pollution (Barking, Essex : 1987)·2020
Same author

Unraveling Polymeric Nanoparticles Cell Uptake Pathways: Two Decades Working to Understand Nanoparticles Journey to Improve Gene Therapy.

Advances in experimental medicine and biology·2020
Same journal

Copper metallodendrimers effectively deliver proapoptotic siRNA to human liver carcinoma HepG2 cells.

International journal of pharmaceutics·2026
Same journal

A styrene-maleic acid copolymer-based boron carrier for tumor-retentive boron delivery in boron neutron capture therapy.

International journal of pharmaceutics·2026
Same journal

Programmable gene modulation networks for Parkinson's disease using nanotechnology enabled CRISPR/Cas brain delivery.

International journal of pharmaceutics·2026
Same journal

Dual carboxylic ionization driven MCC/MAA/IA hydrogels for enhanced oral insulin delivery.

International journal of pharmaceutics·2026
Same journal

Artificial intelligence and CRISPR-based approaches for targeted delivery of bacteriophages.

International journal of pharmaceutics·2026
Same journal

A "three-in-one" nose-to-brain delivery strategy: intranasal vancomycin spray achieves simultaneous clearance of pneumococcal colonization, bacteremia, and meningitis.

International journal of pharmaceutics·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.7K

Development of an optimized freeze-drying protocol for OM-PBAE nucleic acid polyplexes.

C Fornaguera1, C Castells-Sala2, M A Lázaro2

  • 1Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL), Spain.

International Journal of Pharmaceutics
|August 16, 2019
PubMed
Summary
This summary is machine-generated.

This study details critical parameters for freeze-drying oligopeptide end-modified poly (β-aminoester) nanoparticles. Developing a robust lyophilization method enhances the long-term stability of these biodegradable nanoformulations for biomedical use.

Keywords:
Freeze dryingGene deliveryHepes saltNanoformulationNucleic acidsPhosphate buffered salinePoly (β-aminoester) (OM-PBAE)Poly(beta aminoester) polymerPolyplexesSodium acetateStabilitySucrose

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

206.7K

Related Experiment Videos

Last Updated: Jan 20, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.7K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

206.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Polymer Chemistry

Background:

  • Long-term stability of nanoformulations is crucial for biomedical applications.
  • Biodegradable polymers like oligopeptide end-modified poly (β-aminoester) (OM-PBAE) are used for safety but can compromise stability in aqueous dispersions.
  • Freeze-drying (lyophilization) offers a promising method to achieve stable, solid nanoformulations, but the process can induce aggregation.

Purpose of the Study:

  • To investigate critical variables influencing the manufacturing and lyophilization of OM-PBAE nanoparticles.
  • To develop a versatile and robust freeze-drying protocol for preserving a library of OM-PBAE polyplexes with varying pKa values.

Main Methods:

  • Systematic study of parameters affecting OM-PBAE nanoparticle formulation and subsequent freeze-drying.
  • Optimization of lyophilization cycles to mitigate aggregation and ensure nanoparticle integrity.
  • Characterization of freeze-dried OM-PBAE nanoparticles to assess stability and preservation.

Main Results:

  • Identification of key variables that directly impact the success of OM-PBAE nanoparticle lyophilization.
  • Development of a generalized freeze-drying protocol adaptable to different OM-PBAE formulations.
  • Demonstration of enhanced long-term stability for freeze-dried OM-PBAE nanoparticles compared to aqueous dispersions.

Conclusions:

  • Freeze-drying is an effective strategy for enhancing the long-term stability of OM-PBAE nanoparticles.
  • A well-defined lyophilization process is essential for preserving the integrity and functionality of these nanoformulations.
  • The developed protocol provides a versatile approach for stabilizing diverse OM-PBAE polyplexes for biomedical applications.