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Updated: Jan 20, 2026

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
Published on: June 19, 2019
Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells
Sergio López-García1,2, Adrián Lozano3, David García-Bernal1
1Cellular Therapy and Hematopoietic Transplant Unit, Internal Medicine Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30120 Murcia, Spain.
Abstract:
Background: The aim of this study was: to evaluate the biological properties of new hydraulic materials: Bio-C Repair and Bio-C Sealer. Methods: Periodontal ligament stem cells were exposed to several dilutions of Bio-C Repair and Bio-C Sealer. The ion release profile and pH were determined. Metabolic activity, cell migration and cell survival were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing assays and Annexin assays, respectively. Cells were cultured in direct contact with the surface of each material. These were then analyzed via scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Statistical differences were assessed using a two-way ANOVA (α < 0.05). Results: Similar pH was observed in these cements. Bio-C Sealer released significantly more Ca and Si ions (p < 0.05) in comparison with Bio-C Repair. Undiluted Bio-C Sealer induced a significant reduction on cellular viability, cell survival and cell migration when compared to the control (p < 0.05). Moreover, SEM showed abundant cells adhered on Bio-C Repair and a moderate number of cells attached on Bio-C Sealer. Finally, EDX analysis identified higher percentages of Ca and O in the case of Bio-C repair than with Bio-C sealer, while other elements such as Zr and Si were more abundant in Bio-C sealer. Conclusions: Bio-C Repair displayed higher cell viability, cell adhesion and migration rates than Bio-C Sealer.
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