Sonomagnetic Stimulation of Live Cells: Electrophysiologic, Biochemical and Behavioral Responses

Various physical methods have been developed to modulate the electrophysiologic properties of cells and their biochemical signaling pathways. In this study, we propose a sonomagnetic method using pulsed ultrasound (1.1 MHz frequency, 1.1 or 2.2 MPa pressure, 50 cycles per pulse and 500 Hz pulse repetition frequency) and a static magnetic field (680 mT) to stimulate live cells. We found that sonomagnetic stimulation promoted the cell and mitochondrial membrane potentials to more hyperpolarized states. The degree of cell membrane hyperpolarization was cell-type dependent. Furthermore, we found that the intracellular concentrations of Ca²⁺ ions, reactive oxygen species and nitric oxide were substantially increased after sonomagnetic stimulation, and a small decrease in intracellular pH was also observed. Lastly, we found that the daily sonomagnetic stimulation for 3 d inhibited the proliferation rate of neuro-2a cancer cells by 48.64%. Our work demonstrates that sonomagnetic stimulation can effectively perturb cell signaling and drive cancer cells into relatively quiescent states.