Changes in Ca²⁺ Removal Can Mask the Effects of Geometry During IP₃R Mediated Ca²⁺ Signals

Calcium (Ca²⁺) signals are ubiquitous. Most intracellular Ca²⁺ signals involve the release of Ca²⁺ from the endoplasmic reticulum (ER) through Inositol 1,4,5-Trisphosphate Receptors (IP₃Rs). The non-uniform spatial organization of IP₃Rs and the fact that their individual openings are coupled via cytosolic Ca²⁺ are key factors for the variety of spatio-temporal distributions of the cytosolic [Ca²⁺] and the versatility of the signals. In this paper we combine experiments performed in untreated and in progesterone-treated Xenopus laevis oocytes and mathematical models to investigate how the interplay between geometry (the IP₃R spatial distribution) and dynamics (the processes that characterize the release, transport, and removal of cytosolic Ca²⁺) affects the resulting signals. Signal propagation looks more continuous and spatially uniform in treated (mature) than in untreated (immature) oocytes. This could be due to the different underlying IP₃R spatial distribution that has been observed in both cell types. The models, however, show that the rate of cytosolic Ca²⁺ removal, which is also different in both cell types, plays a key role affecting the coupling between Ca²⁺ release sites in such a way that the effect of the underlying IP₃R spatial distribution can be modified.