Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interplasmidic illegitimate recombination in Bacillus subtilis.

V I Bashkirov1, M M Stoilova-Disheva, A A Prozorov

  • 1N.I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.

Molecular & General Genetics : MGG
|August 1, 1988
PubMed
Summary

Illegitimate recombination in Bacillus subtilis involves Staphylococcus aureus plasmids. Recombination primarily occurs at short homologous regions, with some instances at nonhomologous sites, shedding light on plasmid dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methods to study power density distribution in the IVG.1M research reactor after conversion.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2022
Same author

Fungal Planet description sheets: 400-468.

Persoonia·2016
Same author

Fungal Planet description sheets: 320-370.

Persoonia·2015
Same author

[The virulence factors of Mycobacterium tuberculosis: genetic control, new conceptions].

Genetika·2015
Same author

[Systems of genes and proteins affecting mycobacteria virulence and their homologs participation in conjugation of mycobacterium smegmatis].

Genetika·2013
Same author

[Mycobacterium tuberculosis mutants with multidrug resistance: history of origin, genetic and molecular mechanisms of resistance, and emerging challenges].

Genetika·2012

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Bacterial plasmids, such as Staphylococcus aureus pE194 and Escherichia coli pBR322, can undergo recombination.
  • Understanding illegitimate recombination is crucial for bacterial genetics and plasmid stability.

Purpose of the Study:

  • To investigate illegitimate recombination between specific Staphylococcus aureus plasmids in Bacillus subtilis.
  • To characterize the types and mechanisms of recombinant DNA junctions formed.

Main Methods:

  • Utilized Bacillus subtilis as a host for studying plasmid recombination.
  • Generated cointegrates between plasmids pE194 and pBD17.
  • Analyzed 22 hybrid recombinants using nucleotide sequencing of DNA junctions.

Related Experiment Videos

Main Results:

  • Illegitimate recombination occurred at a frequency of 1-3 x 10(-8).
  • Nine distinct types of recombinants were identified among the analyzed hybrids.
  • Recombination predominantly occurred at short homologous regions (9-15 bp) in 8 cases, with one instance at nonhomologous sites.
  • Recombination sites showed similarity to those involved in pE194 chromosomal integration.

Conclusions:

  • Illegitimate recombination between studied plasmids involves both homologous and nonhomologous mechanisms.
  • The findings suggest shared mechanisms between plasmid recombination and chromosomal integration.
  • Further research into illegitimate recombination mechanisms is warranted.