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Protein compounds of Bacillus subtilis with in vitro antifungal activity against Pseudocercospora fijiensis (Morelet)

Mileidy Cruz-Martín1, Eilyn Mena2, Mayra Acosta-Suárez2

  • 1Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5., CP 54830, Santa Clara, Villa Clara, Cuba. mileidy@ibp.co.cu.

Brazilian Journal of Microbiology : [Publication of the Brazilian Society for Microbiology]
|August 17, 2019
PubMed

Insights

Bacillus subtilis CCIBP-M27 metabolites show antifungal activity against Pseudocercospora fijiensis by inhibiting conidial germination and mycelial growth. Peptide compounds in the filtrate cause observable cellular damage in the fungus.

Area of Science:

  • Agricultural Science
  • Microbiology
  • Plant Pathology

Background:

  • * Pseudocercospora fijiensis causes significant crop losses globally.
  • * Bacillus subtilis is known for its beneficial properties, including biocontrol potential.
  • * Understanding microbial metabolites is crucial for developing sustainable agricultural solutions.

Purpose of the Study:

  • * To evaluate the antifungal potential of Bacillus subtilis CCIBP-M27 metabolites against Pseudocercospora fijiensis.
  • * To investigate the mechanism of action of these metabolites on fungal growth and morphology.
  • * To identify the types of compounds responsible for the observed antifungal effects.

Main Methods:

  • * Preparation and application of Bacillus subtilis CCIBP-M27 culture filtrate.
  • * Assays for ascospore and conidial germination inhibition.
  • * Measurement of mycelial growth inhibition.
  • * Microscopic analysis of fungal morphology after treatment.

Main Results:

  • * Culture filtrate significantly reduced conidial germination and mycelial growth of Pseudocercospora fijiensis.
  • * Ascospore germination was not inhibited by the culture filtrate.
  • * Microscopic examination revealed vacuolization and swelling in P. fijiensis mycelia upon exposure to the filtrate.
  • * Peptide-type compounds isolated from the filtrate induced similar cellular deformations.

Conclusions:

  • * Bacillus subtilis CCIBP-M27 produces metabolites with significant in vitro antifungal activity against Pseudocercospora fijiensis.
  • * The antifungal effect is primarily mediated by protein or peptide substances present in the culture filtrate.
  • * These metabolites show potential as biocontrol agents for managing fungal diseases in agriculture.
Keywords:
BiocontrolBlack SigatokaMetabolitesProteins

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