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Published on: March 29, 2018
In Vitro Evaluation of Recombinant Bone Morphogenetic Protein-2 Bioactivity for Regenerative Medicine
Stephanie L Fung1, Xiaohuan Wu1, Julian P Maceren1
1New Jersey Center for Biomaterials, Rutgers-The State University of New Jersey, Piscataway, New Jersey.
Impact Statement:
This work is a systematic evaluation of the experimental parameters of the most widely used in vitro recombinant human bone morphogenetic protein-2 (rhBMP-2) activity assays. The variations in assays reported in the literature have challenged the reproducibility and translation of work using rhBMP-2 as a bone-inducing growth factor. By elucidating the effect of model cell line on the dose-dependent alkaline phosphatase response to rhBMP-2 induction and by establishing a correlation between protein activity and protein concentration by enzyme-linked immunosorbent assay using commercially available rhBMP-2, this work is a significant step toward developing an in vitro-in vivo correlation between quantified activity and clinical efficacy.
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