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Updated: Jan 20, 2026
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Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
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Sphingomonas gilva sp. nov., isolated from mountain soil
Dahui Zhu1, Yaxin Niu1, Dongmei Liu1
1State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Summary
A novel yellow, rod-shaped bacterium, Sphingomonas gilva sp. nov. strain ZDH117T, was discovered in Chinese soil. This discovery expands the known diversity within the Sphingomonas genus.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- The genus Sphingomonas comprises diverse Gram-negative bacteria with significant ecological and biotechnological roles.
- Soil environments are rich reservoirs of microbial diversity, including novel bacterial species.
- Accurate taxonomic classification is crucial for understanding microbial communities and their functions.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from a soil sample collected in Guangdong Province, China.
- To determine the taxonomic position of the novel strain within the genus Sphingomonas using polyphasic characterization.
- To propose a new species designation for the novel isolate based on comprehensive analysis.
Main Methods:
- Isolation and cultivation of bacteria from soil samples.
- Gram staining, aerobic growth, motility, and pigment analysis.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Genomic DNA G+C content determination.
- Chemotaxonomic analyses including respiratory quinones, polyamines, cellular fatty acids, and polar lipids.
Main Results:
- A novel Gram-negative, aerobic, motile, yellow, rod-shaped bacterium, strain ZDH117T, was isolated.
- Phylogenetic analysis based on 16S rRNA gene sequences showed highest similarity to Sphingomonas adhaesiva, but clustered with Sphingomonas zeicaulis.
- Genomic average nucleotide identity (ANI) values were below the threshold for species delineation with related species.
- Chemotaxonomic data (respiratory quinone, polyamine, fatty acids, polar lipids) were consistent with the genus Sphingomonas.
- The G+C content of the genomic DNA was 67.6 mol%.
Conclusions:
- Strain ZDH117T represents a distinct genotypic and phenotypic lineage within the genus Sphingomonas.
- Based on integrated polyphasic taxonomic data, strain ZDH117T is proposed as a novel species, Sphingomonas gilva sp. nov.
- The proposed type strain is ZDH117T (=KCTC 62894T=CCTCCAB 2018262T).
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