Related Experiment Video

Updated: Jan 20, 2026

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

17.1K

Determination of high-sensitivity cardiac troponin T upper reference limits under the improved selection criteria in

Xin Zhang1,2, Xiaoxu Han1,2, Min Zhao1,2

  • 1Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China.

Journal of Clinical Laboratory Analysis
|August 17, 2019
PubMed

Insights

Establishing accurate reference limits for high-sensitivity cardiac troponin T (hs-cTnT) is crucial. Improved selection criteria for healthy populations significantly reduce hs-cTnT upper reference limits, avoiding overestimation.

Area of Science:

  • Clinical Chemistry
  • Cardiovascular Diagnostics
  • Biomarker Reference Limits

Background:

  • Lack of consensus exists on defining reference populations for high-sensitivity cardiac troponin (hs-cTn) upper reference limits (URL).
  • Accurate determination of hs-cTn URLs is essential for reliable clinical interpretation.
  • Previous criteria may not adequately exclude individuals with subclinical cardiovascular risk factors.

Purpose of the Study:

  • To establish the 99th percentile upper reference limits (URLs) for high-sensitivity cardiac troponin T (hs-cTnT).
  • To compare URLs derived from 2018 AACC/IFCC criteria versus improved selection criteria.
  • To evaluate the impact of excluding hypertension, overweight/obesity, and dyslipidemia on hs-cTnT URLs.

Main Methods:

  • Stratified cluster sampling of 1848 apparently healthy individuals in Shenyang, China.
  • Application of 2018 AACC/IFCC criteria using surrogate biomarkers.
  • Implementation of improved selection criteria including physical examination and laboratory screening to exclude hypertension, overweight/obesity, and dyslipidemia.

Main Results:

  • Using 2018 AACC/IFCC criteria, the 99th percentile URLs for hs-cTnT were 19 ng/L (male), 16 ng/L (female), and 18 ng/L (total).
  • Improved selection criteria resulted in lower 99th percentile URLs: 18 ng/L (male), 13 ng/L (female), and 16 ng/L (total).
  • Male hs-cTnT URLs were consistently higher than female URLs across all age groups.

Conclusions:

  • Improved selection criteria, incorporating questionnaire, physical examination, and laboratory screening, are effective in refining the reference population.
  • These enhanced criteria help to avoid overestimation of the 99th percentile upper reference limit for hs-cTnT.
  • The findings underscore the importance of rigorous population selection for accurate hs-cTnT biomarker interpretation.
Abstract

Keywords:
99th percentilehigh-sensitivity cardiac troponin Treference populationselection criteria

More Related Videos

Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training07:40

Impact of High-intensity Interval Exercise and Moderate-Intensity Continuous Exercise on the Cardiac Troponin T Level at an Early Stage of Training

Published on: October 10, 2019

7.7K
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos09:42

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Published on: October 12, 2017

10.2K

Related Concept Videos

Limits to Natural Selection01:38

Limits to Natural Selection

Organisms that are well-adapted to their environment are more likely to survive and reproduce. However, natural selection does not lead to perfectly adapted organisms. Several factors constrain natural selection.
34.1K
Population Growth00:57

Population Growth

Population size is dynamic, increasing with birth rates and immigration, and decreasing with death rates and emigration. In ideal conditions with unlimited resources, populations can increase exponentially, which plots as a J-shaped growth rate curve of population size against time. This type of curve is characteristic of newly-introduced invasive species, or populations that have suffered catastrophic declines and are rebounding.
27.9K
Criteria for Causality: Bradford Hill Criteria - II01:28

Criteria for Causality: Bradford Hill Criteria - II

The Bradford Hill criteria serve as guidelines for establishing causative links in epidemiological research. Beyond Strength, Consistency, Specificity, and Temporality, key criteria also include Biological Gradient, Plausibility, Coherence, Experiment, and Analogy. These principles assist scientists in assessing the likelihood of causation in complex biological contexts. Below is a summary of these concepts:
1.2K
Criteria for Causality: Bradford Hill Criteria - I01:30

Criteria for Causality: Bradford Hill Criteria - I

The Bradford Hill criteria are a group of principles that provide a framework to determine a causal relationship between a specific factor and a disease. There are nine criteria that are pivotal in assessing causality in epidemiological studies. Here's a closer look at Strength, Consistency, Specificity, and Temporality criteria with definitions and examples:
1.1K
What is Population Genetics?01:25

What is Population Genetics?

A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.5K
Limiting Reactant02:27

Limiting Reactant

The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in reality, the reactants are not always present in the stoichiometric amounts indicated by the balanced equation.
69.4K