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Published on: June 9, 2011
Colorimetric and Fluorimetric DNA Detection with a Hydroxystyryl-Quinolizinium Photoacid and Its Application for Cell
Avijit Kumar Das1, Sergey I Druzhinin1, Heiko Ihmels1
1Department of Chemistry and Biology, and Center of Micro- and Nanochemistry and Engineering (Cμ), University of Siegen, Adolf-Reichwein-Str. 2, 57068, Siegen, Germany.
Abstract:
The combination of styryl dye properties with the acidity and strong photoacidity of the 2,2'-[(1''-hydroxy-4''-methyl-(E)-2'',6''-phenylene)]-bisquinolizinium enables the detection of DNA by distinct absorption and emission color changes and the fluorimetric detection of DNA in cells with epifluorescence and confocal fluorescence microscopy.
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