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Pterostilbene Inhibits Adipocyte Conditioned-Medium-Induced Colorectal Cancer Cell Migration through Targeting

Yu-Hsuan Hsiao1, Nien-Chi Chen1, Yen-Chun Koh1

  • 1Institute of Food Sciences and Technology , National Taiwan University , No. 1, Sec. 4, Roosevelt Road , Taipei 106 , Taiwan.

Journal of Agricultural and Food Chemistry
|August 17, 2019
PubMed

Insights

Pterostilbene (PTS) inhibits colorectal cancer (CRC) metastasis by downregulating fatty acid-binding protein 5 (FABP5) and key signaling pathways. This phenolic compound offers potential for managing obesity-related CRC progression.

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Obesity is a risk factor for colorectal cancer (CRC) progression and metastasis.
  • Pterostilbene (PTS), a phenolic compound, exhibits various pharmacological activities but its role in obesity-related CRC is not well understood.

Purpose of the Study:

  • To investigate the mechanism by which PTS inhibits adipocyte-conditioned medium (aCM)-induced malignant transformation and metastasis in HT-29 colorectal adenocarcinoma cells.

Main Methods:

  • Cells were treated with aCM and PTS.
  • Expression of FABP5, prometastatic factors (VEGF, MMP2, MMP9, TNFα), and signaling pathway proteins (NF-κB, β-catenin, PPAR-γ, PI3K/Akt, MAPK/ERK, JNK) were analyzed.

Main Results:

  • PTS downregulated aCM-induced FABP5 and prometastatic factors (VEGF, MMP2, MMP9, TNFα).
  • PTS inhibited activation of NF-κB, β-catenin, and PPAR-γ pathways.
  • PTS suppressed upstream signaling pathways including PI3K/Akt, p38 MAPK, ERK, and JNK 1/2.

Conclusions:

  • Pterostilbene exhibits anti-metastatic properties in colorectal cancer by inhibiting FABP5 expression and multiple oncogenic signaling pathways.
  • PTS may serve as a potential therapeutic agent to counteract obesity-induced CRC metastasis.
Keywords:
adipocyte conditioned-medium (aCM)colorectal cancer (CRC)fatty acid-binding protein 5 (FABP5)obesitypterostilbene (PTS)

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