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Rosette formation by buffalo (Bos bubalis) T and B lymphocytes
The Veterinary Record
|April 28, 1979
Summary
This study identifies buffalo T cells using sheep red blood cell E rosettes (17.7%) and buffalo B cells using sheep red blood cell-antibody-complement (SRBC-EAC) rosettes (5-18.5%). These findings aid in understanding buffalo immunology.
Area of Science:
- Immunology
- Veterinary Science
- Cell Biology
Background:
- Buffalo (Bos bubalis) immune cell identification is crucial for veterinary research.
- Distinguishing T and B lymphocytes is fundamental in immunology.
Purpose of the Study:
- To establish reliable methods for detecting buffalo T and B lymphocytes.
- To utilize rosette formation assays for buffalo lymphocyte characterization.
Main Methods:
- Purification of buffalo lymphocytes.
- E rosette assay using sheep erythrocytes (SRBC) to identify T cells.
- EAC rosette assay using SRBC-antibody-complement to identify B cells.
Main Results:
- Sheep erythrocytes formed 17.7% E rosettes with buffalo lymphocytes, indicating T cells.
- Other erythrocytes showed lower rosette formation.
- 5-18.5% EAC rosettes were observed with buffalo lymphocytes, indicating B cells.
Conclusions:
- E rosette formation with SRBC is a viable marker for buffalo T cells.
- EAC rosette formation with SRBC is a viable marker for buffalo B cells.
- This study provides a basis for further immunological studies in buffalo.