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Extraction of protective components of Rickettsia rickettsii with n-octyl-beta-D-glucopyranoside
G A McDonald1, R L Anacker, R E Mann
1Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Reviews of Infectious Diseases
|July 1, 1988
Abstract:
Two Rickettsia rickettsii proteins, with apparent molecular masses of 120 and 155 kilodaltons, have been implicated as important antigens in immunity to Rocky Mountain spotted fever. An n-octyl-beta-D-glucopyranoside-soluble fraction of R. rickettsii was found to be enriched for these proteins along with others. Vaccination of mice with the detergent-soluble fraction protected them against death caused by R. rickettsii and protected guinea pigs from developing Rocky Mountain spotted fever after being challenged with viable R. rickettsii.