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Updated: Jan 20, 2026

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Metagenomic approaches: effective tools for monitoring the structure and functionality of microbiomes in anaerobic
Annerys Carabeo-Pérez1, Gilda Guerra-Rivera2, Miguel Ramos-Leal3
1Centro de Estudios de Energía y Procesos Industriales, Universidad de Sancti Spíritus "José Martí Pérez", Ave de los Mártires No. 360, CP 60100, Sancti Spíritus, Cuba.
Abstract:
Microbial metagenome analysis has proven its usefulness to investigate the microbiomes present in technical engineered ecosystems such as anaerobic digestion systems. The analysis of the total microbial genomic DNA allows the detailed determination of both the microbial community structure and its functionality. In addition, it enables to study the response of the microbiome to alterations in technical process parameters. Strategies of functional microbial networks to face abiotic stressors, e.g., resistance, resilience, and reorganization, can be evaluated with respect to overall process optimization. The objective of this paper is to review the main metagenomic tools used for effective studies on anaerobic digestion systems in monitoring the dynamic of the microbiomes, as well as the factors that have been identified so far as limiting the metagenomic studies in this ecosystems.
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