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Immunopurification of human factor VIII/vWF complex from plasma
Thrombosis and Haemostasis
|June 16, 1988
Summary
This study presents a novel immunopurification method for the coagulation factor FVIII/vWF complex directly from plasma, achieving high recovery and stability for therapeutic applications.
Area of Science:
- Biochemistry
- Immunology
- Hematology
Background:
- The FVIII/vWF complex is crucial for hemostasis.
- Efficient purification methods are needed for therapeutic applications.
Purpose of the Study:
- To develop a direct plasma immunopurification process for the active FVIII/vWF complex.
- To optimize antibody binding and elution conditions for high recovery and purity.
Main Methods:
- Selection of a monoclonal antibody (mAb) against von Willebrand factor (vWF).
- Immobilization of the mAb onto Sephacryl S-1000 using benzoquinone coupling.
- Development of a purification process from plasma under physiologic conditions.
Main Results:
- Achieved 50% recovery of both FVIII and vWF activities.
- Produced an enriched product with high specific activity (20 IU/mg protein).
- The purified complex demonstrated stability after lyophilization.
Conclusions:
- The developed immunopurification process is effective for obtaining active FVIII/vWF complex from plasma.
- The method offers high recovery, purity, and stability, suitable for further development.