Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.9K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.9K
Epigenetic Regulation01:46

Epigenetic Regulation

33.5K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.5K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.4K
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

30.6K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
30.6K
Genetics of Speciation02:16

Genetics of Speciation

20.9K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
20.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.8K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different goals, common ground: A global perspective on dairy breeding objectives.

JDS communications·2026
Same author

Liver transcriptome analysis reveals sex-dependent genes and gene networks linked to carcass traits in suckling lambs.

Animal : an international journal of animal bioscience·2026
Same author

Integrating computed tomography and deep learning to assess tibiotarsus cortical bone area for leg health evaluation in purebred male turkeys.

Animal : an international journal of animal bioscience·2026
Same author

Novel alternative splicing associated with feed efficiency in dairy cattle under distinct diet compositions.

Journal of dairy science·2026
Same author

Invited review: Global census on the development and implementation of direct genetic selection for enteric methane emissions in dairy cattle.

Journal of dairy science·2026
Same author

Genetic correlation analyses of serum biochemical variables with health, fertility, and production traits in Holstein cows.

Journal of dairy science·2026

Related Experiment Video

Updated: Jan 20, 2026

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Published on: February 22, 2019

11.2K

Genetic mechanisms regulating the host response during mastitis.

V Asselstine1, F Miglior1, A Suárez-Vega1

  • 1Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.

Journal of Dairy Science
|August 19, 2019
PubMed
Summary

This study analyzed Holstein cow milk cells to identify genes involved in mastitis resistance. Researchers found specific immune-related genes that could help breed cows less susceptible to this costly dairy disease.

Keywords:
RNA-Sequencingdairy cattlemastitismilk somatic cell

More Related Videos

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

6.9K
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.4K

Related Experiment Videos

Last Updated: Jan 20, 2026

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Published on: February 22, 2019

11.2K
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

6.9K
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.4K

Area of Science:

  • * Dairy Cattle Genetics
  • * Animal Immunology
  • * Molecular Biology

Background:

  • * Mastitis is a significant economic and health challenge in the dairy industry.
  • * Understanding host response to intramammary infections is crucial for improving dairy cow health.
  • * Transcriptome analysis offers insights into the genetic basis of disease resistance.

Purpose of the Study:

  • * To characterize the Holstein milk somatic cell transcriptome in healthy versus mastitic quarters.
  • * To identify differentially expressed genes and enriched metabolic pathways related to mastitis.
  • * To pinpoint candidate genes within known quantitative trait loci associated with mastitis resistance.

Main Methods:

  • * RNA-Sequencing was performed on milk somatic cells from healthy and mastitic quarters of 6 Holstein cows.
  • * Differential gene expression analysis was conducted using a false discovery rate <0.05 and fold change >±2.
  • * In silico functional analysis identified significantly enriched metabolic pathways (false discovery rate <0.01).

Main Results:

  • * 449 genes were differentially expressed between healthy and mastitic quarters.
  • * Key genes associated with milk components (CSN2, CSN3) were highly expressed in healthy quarters, while immune genes (B2M, CD74) dominated in mastitic quarters.
  • * Enriched pathways included cytokine-cytokine interaction and cell adhesion molecules; seven candidate genes were identified.

Conclusions:

  • * Multiple genes within quantitative trait loci regions may influence host susceptibility to mastitis.
  • * Identified candidate genes offer potential for marker-assisted selection to enhance mastitis resistance in dairy cattle.
  • * This research contributes to understanding the genetic architecture of mastitis, aiding sustainable agricultural practices.