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Differential effects of selective serotonin reuptake inhibitors on paraoxonase-1 enzyme activity: An in vitro study

Yeliz Demir¹, Neslihan Balcı², Mahmut Gürbüz³

¹Department of Chemistry, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey; Department of Pharmacy Services, Nihat Delibalta Göle Vocational High School, Ardahan University, 75700, Ardahan, Turkey.

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP

|August 19, 2019

Abstract:

Paraoxonase-I (PON1) is a calcium-dependent hydrolytic enzyme, plays an important role in most antioxidant properties related to high-density lipoprotein (HDL). Antidepressant drugs are commonly employed in treatment of mood disorders and anxiety treatment. In this study, human serum PON1 was purified using simple reproducible procedures and the effects of some antidepressant drugs on its activity were determined. It was found that mirtazapine, aripiprazole, escitalopram, and risperidone exhibited potential inhibitory properties on the purified PON1 activity with IC₅₀ values in the range of 115.50-231.00 μM and K_i values in the range of 41.66 ± 4.27 μM-276.36 ± 35.28 μM. Both risperidone and escitalopram inhibited PON1 activity competitively, while both aripiprazole and mirtazapine inhibited PON1 activity non-competitively. Chlorpromazine did not affect PON1 activity. Usage of drugs with significant biological activity may be hazardous in some cases.

Keywords:

Antidepressant drugs Enzyme inhibition Enzyme purification Oxidative stress Paraoxonase

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