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Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading
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Representative subsampling of sedimenting blood.

Bhargav Rallabandi1,2, Janine K Nunes2, Antonio Perazzo2

  • 1Department of Mechanical Engineering, University of California, Riverside, CA 92521, USA.

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|August 20, 2019
PubMed
Summary
This summary is machine-generated.

Accurate blood subsampling is challenging due to cell sedimentation. This study presents a Kynch sedimentation model to ensure representative blood subsampling and offers methods to concentrate or dilute blood samples.

Keywords:
Krieger–DoughertyKynchbloodcomplex fluidssedimentation

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Area of Science:

  • Biomedical Engineering
  • Fluid Dynamics
  • Hematology

Background:

  • Extracting small blood volumes for diagnostics is crucial.
  • Cellular sedimentation in blood complicates representative subsampling.
  • Physiological hematocrit ranges present a challenge for accurate sampling.

Purpose of the Study:

  • To develop a model for representative blood subsampling.
  • To address challenges posed by rapid blood cell sedimentation.
  • To design a method for controlled blood subsampling.

Main Methods:

  • Utilized a Kynch sedimentation model.
  • Incorporated experimental data for model validation.
  • Analyzed design considerations for constant cross-sectional area containers.

Main Results:

  • Developed a model ensuring representative blood subsampling across hematocrit ranges.
  • Demonstrated a method to exploit sedimentation for subsampling.
  • Showcased the ability to achieve higher or lower hematocrit in subsamples.

Conclusions:

  • The Kynch sedimentation model provides a tool for accurate blood subsampling.
  • The method allows for controlled concentration or dilution of blood samples.
  • This approach is applicable to other sedimenting suspensions beyond blood.