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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
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Long non-coding RNA HAND2-AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma

Shunguang Chen1, Xiaoming Xu1, Shengjun Lu1

  • 1Department of Orthopedics, Jingzhou Central Hospital, Jingzhou, Hubei 434020, P.R. China.

Oncology Letters
|August 20, 2019
PubMed

Insights

Long non-coding RNA HAND2-AS1 (lncRNA HAND2-AS1) acts as a tumor suppressor in osteosarcoma. Reduced HAND2-AS1 levels promote cancer cell proliferation by increasing glucose metabolism, suggesting its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Long non-coding RNA heart and neural crest derivatives expressed 2-antisense RNA 1 (lncRNA HAND2-AS1) is a known tumor suppressor in endometrial carcinoma.
  • The role of lncRNA HAND2-AS1 in osteosarcoma remains unexplored.

Purpose of the Study:

  • To investigate the expression and function of lncRNA HAND2-AS1 in osteosarcoma.
  • To determine the relationship between lncRNA HAND2-AS1 and osteosarcoma progression.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to detect HAND2-AS1 expression in tumor tissues and serum.
  • Transfection of osteosarcoma cells with HAND2-AS1 small interfering RNA (siRNA).
  • Cell Counting Kit-8, western blotting, and glucose uptake assays to assess cell proliferation, glucose transporter 1 (GLUT1) expression, and glucose uptake.

Main Results:

  • HAND2-AS1 expression was significantly reduced in osteosarcoma tissues and serum compared to healthy controls.
  • Lower serum HAND2-AS1 levels correlated with larger tumor size.
  • HAND2-AS1 knockdown promoted osteosarcoma cell proliferation, increased glucose uptake, and upregulated GLUT1 expression.

Conclusions:

  • lncRNA HAND2-AS1 functions as a tumor suppressor in osteosarcoma.
  • HAND2-AS1 may inhibit osteosarcoma cell proliferation by regulating glucose metabolism via GLUT1.
  • Serum HAND2-AS1 levels could serve as a potential biomarker for osteosarcoma tumor size.
Keywords:
glucose transporter 1glucose uptakelong non-coding RNA HAND2-AS1osteosarcoma

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