Related Experiment Video

Updated: Jan 20, 2026

Culture of Bladder Cancer Organoids as Precision Medicine Tools
08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

5.4K

Interim analysis of a real-world precision medicine platform for molecular profiling of metastatic or advanced

Markus Kieler1, Matthias Unseld1, Daniela Bianconi1

  • 1Department of Medicine I, Division of Oncology, Medical University of Vienna, Wien, Austria.

ESMO Open
|August 20, 2019
PubMed
Abstract

Insights

Precision medicine identifies actionable mutations for targeted cancer therapy. While effective, integrating molecular profiling into advanced cancer treatment presents significant challenges for oncologists.

Area of Science:

  • Oncology
  • Genomics
  • Precision Medicine

Background:

  • High-throughput genomic profiling aids in identifying actionable mutations for personalized cancer therapy.
  • The clinical application of molecular profiling is increasing, but challenges remain in interpreting results and guiding targeted treatments for advanced cancers.

Purpose of the Study:

  • To evaluate the utility of a precision medicine platform (MONDTI) for molecular characterization of metastatic solid tumors.
  • To identify actionable genomic alterations and guide individualized targeted therapy.

Main Methods:

  • Comprehensive molecular profiling of tumor specimens from 295 patients with metastatic solid tumors (2013-2016).
  • Analysis included mutational profiling, immunohistochemistry (IHC), and cytogenetics.
  • Results and treatment suggestions were discussed in multidisciplinary tumor board meetings.

Main Results:

  • Mutational profiles were obtained for 99% of patients. Common mutations included TP53 (39%), KRAS (19%), and PIK3CA (9.5%).
  • IHC revealed frequent positivity for phospho-mTOR (71%), EGFR (68%), MET (56%), and PDGFRα (48%).
  • Targeted therapy recommendations were made for 55.6% of samples with detected alterations, leading to individualized treatment concepts in 24% of cases.

Conclusions:

  • An open approach to molecular profiling and immunotherapy for solid tumors can facilitate individualized treatment strategies.
  • Implementing molecularly matched therapies for patients with advanced cancer remains a complex challenge.
Keywords:
precision medicine – molecular profile – targeted treatment

More Related Videos

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

9.1K
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

11.5K

Related Concept Videos

Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
1.3K
Real Gases: Effects of Intermolecular Forces and Molecular Volume Deriving Van der Waals Equation04:01

Real Gases: Effects of Intermolecular Forces and Molecular Volume Deriving Van der Waals Equation

Thus far, the ideal gas law, PV = nRT, has been applied to a variety of different types of problems, ranging from reaction stoichiometry and empirical and molecular formula problems to determining the density and molar mass of a gas. However, the behavior of a gas is often non-ideal, meaning that the observed relationships between its pressure, volume, and temperature are not accurately described by the gas laws.
38.8K
Cancer Survival Analysis01:21

Cancer Survival Analysis

Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...
650
Uncertainty in Measurement: Accuracy and Precision03:37

Uncertainty in Measurement: Accuracy and Precision

Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value. 
100.1K
Accuracy and Precision01:52

Accuracy and Precision

Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.  Highly accurate...
14.2K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
998