Chryseobacterium mulctrae sp. nov., isolated from raw cow's milk

A Gram-stain-negative bacterial strain, designated CA10^T, was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10^T belonged to the genus Chryseobacterium, family Flavobacteriaceae, and was most closely related to Chryseobacterium indoltheticum ATCC 27950^T (98.75 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values of strain CA10^T were 94.4 and 56.9 %, respectively, relative to Chryseobacterium scophthalmum DSM 16779^T, being lower than the cut-off values of 95-96 and 70 %, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C_15 : 0, summed feature 9 (iso-C_17 : 1ω9c and/or C_16 : 0 10-methyl), summed feature 3 (iso-C_15 : 0 2-OH and/or C_16 : 1ω7c) and iso-C_17 : 0 3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10^T is a novel species of genus Chryseobacterium, for which the name Chryseobacterium mulctrae sp. nov. is proposed. The type strain is CA10^T (=KACC 21234^T=JCM 33443^T).