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Updated: Jan 20, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Yeast As a Chassis for Developing Functional Assays to Study Human P53.

Paola Monti¹, Bartolomeo Bosco², Sara Gomes³

¹Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino.

Journal of Visualized Experiments : Jove

|August 20, 2019

Summary

Mammalian P53 protein functions as a transcription factor in yeast, enabling novel assays. These assays study P53 binding sites, mutations, and cofactor impacts on gene activation.

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Area of Science:

Molecular Biology
Yeast Genetics
Transcription Factor Research

Background:

The mammalian P53 protein is a crucial transcription factor (TF) involved in cellular responses.
Studying P53 function in mammalian systems can be complex due to cellular context and genetic background.
Yeast (S. cerevisiae) offers a simplified model system for investigating transcription factor activity.

Purpose of the Study:

To review and assess yeast-based functional assays for studying P53 transcription factor activity.
To highlight the utility of yeast for investigating P53 binding site specificity, mutation effects, and cofactor interactions.
To explore the application of these assays for basic and translational research, including related TFs like P63 and P73.

Main Methods:

Utilizing yeast (S. cerevisiae) as a model organism for P53 functional studies.
Employing genome editing in isogenic yeast strains to create reporter systems for P53 response elements (REs).
Leveraging regulated systems for ectopic P53 expression to evaluate transactivation across various protein levels.
Reviewing reporter systems based on color genes, luciferase, and yeast growth.

Main Results:

Yeast functional assays provide a powerful platform to study P53 transactivation specificity.
These systems allow for the assessment of TP53 mutations, cofactors, and small molecule impacts on P53 activity.
The ease of genome editing and regulated P53 expression in yeast facilitates versatile experimental designs.

Conclusions:

Yeast-based assays offer significant advantages for studying P53 transcription factor function and specificity.
These methods are adaptable for evaluating the effects of genetic variations, mutations, and therapeutic compounds.
The versatility extends to studying other members of the TP53 gene family, such as P63 and P73.