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Related Experiment Video

Updated: Jan 20, 2026

Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function
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Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function.

Yuji Matsukawa1, Yuki Ide2, Kazuo Umemura2

  • 1Department of Physics, Graduate School of Science, Tokyo University of Science; 2214101@alumni.tus.ac.jp.

Journal of Visualized Experiments : Jove
|August 20, 2019
PubMed
Summary

A novel device secures sample containers for vertical microscopy, enabling gravity and temperature-dependent behavior studies. Remote monitoring of temperature is also supported.

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Area of Science:

  • Microscopy
  • Biophysics
  • Materials Science

Background:

  • Standard microscopy stages are horizontal, limiting observation of gravity-influenced or buoyant sample behaviors.
  • Observing dynamics in the vertical plane requires specialized equipment and sample manipulation.

Purpose of the Study:

  • To develop a device for securing sample containers on a vertically oriented microscope stage.
  • To enable the study of gravity-dependent sample dynamics and afloat behavior.
  • To facilitate temperature-controlled observations of sample behavior.

Main Methods:

  • A sideways inverted microscope tilted by 90° was utilized.
  • A newly developed device was used to secure sample containers (Petri dishes, glass slides) vertically.
  • A silicone rubber heater was integrated for temperature regulation.

Main Results:

  • The device successfully secured sample containers on the vertical stage, allowing for observation of dynamics in the vertical plane.
  • Temperature regulation enabled the study of temperature-dependent sample behaviors.
  • Temperature data was transferred to an internet server for remote monitoring and control.

Conclusions:

  • The developed device is effective for vertical sample observation, expanding microscopy capabilities.
  • The system allows for remote, controlled observation of temperature-dependent phenomena in a vertical orientation.
  • This setup is valuable for studying gravity and temperature effects on various sample types.