Related Experiment Video
Updated: Jan 20, 2026
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
Published on: July 19, 2019
Programmed cell death-1 in patients with primary liver cancer and its effect on prognosis
Min Yang1, Wen Wen Dou, Guang Hong Sun
1Department of Infectious Diseases, Affiliated Hospital of Weifang Medical University, Weifang 261031, P.R. China.
Purpose:
To detect the expression of programmed cell death-1 (PD-1) in peripheral blood T lymphocytes of patients with primary liver cancer and its effect on their prognosis.
Methods:
The medical records of 42 patients with primary liver cancer, 36 patients with chronic hepatitis B (CHB) and 38 healthy volunteers composed the liver cancer group, benign lesion group and control group, respectively. Fluorescence quantitative RT-PCR (qRT-PCR) was used for detecting the expression level of PD-1 mRNA in peripheral blood T lymphocytes of subjects in the three groups, and flow cytometry was used for detecting the positive expression of PD-l protein on the surface of T lymphocytes of subjects.
Results:
Patients in the control group and the benign lesion group had lower expression level of PD-1 mRNA than those in the liver cancer group (p<0.05). Patients in the control group had lower expression level of PD-1 mRNA than those in the benign lesion group (p<0.05). Patients in the control group and the benign lesion group had lower positive expression rate of PD-1 protein on the surface of T lymphocytes than those in the liver cancer group (p<0.05).
Conclusion:
The expression of PD-1 in the peripheral blood was higher in patients with primary liver cancer. Patients in the PD-1 low expression group had significantly better prognosis than those in the PD-1 high expression group. PD-1 may be related to the occurrence and development of primary liver cancer and is worthy of further study.
More Related Videos
Related Concept Videos
01:30Overview of Cell Death
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
01:18Autophagic Cell Death
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
01:27Liver Histology
Hepatocytes perform a variety of essential functions. They secrete...
01:30Liver Physiology
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
01:35Cancer Cell Migration through Invadopodia
02:53Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...