Related Experiment Video
Updated: Jan 20, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
Published on: March 14, 2014
The effect of the overexpression of miR-490 on the biological function of prostate cancer cell PC-3
Yingbo Hu1, Zhengdong Hong, Cheng Cheng
1Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, P.R.China.
Purpose:
To investigate the effect and molecular mechanism of miR-490 in prostate cancer.
Methods:
qRT-PCR was used to detect the expression of miR-490 in human prostate cancer PC-3 cell line (the control group B) and normal human prostate cells (the control group A), the overexpression vector (the positive group) and human prostate cancer PC-3 cells transfected by negative control (the negative group) were built, and the results of the transfection were detected by qRT-PCR.
Results:
The relative expression levels of miR-490 in the cells in the control group A, the control group B and the negative group were lower than that in the positive group (p<0.05). The OD values of the cells in the negative and positive groups both increased continuously over time (p<0.05). The OD values of the cells in the positive group at each time point were lower than those in the negative group (p<0.05).
Conclusion:
The low expression and overexpression of miR-490 in prostate cancer may inhibit the proliferation, migration and invasion of prostate cancer cells and promote apoptosis by inhibiting the phosphorylation of AKT signal pathway.
Related Concept Videos
What is Conservation Biology?
Biological Effects of Radiation
Synthetic Biology
Golden rice
Golden rice is a genetically modified...
Biological Causes of Schizophrenia
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cancer Cell Migration through Invadopodia

