Age-specific effects of P2X7 receptors on olfactory function in mice

This study aimed to explore the age-specific effects of P2X7 receptor (P2X7R) knockout on olfactory function in mice. In this study, we analyzed olfactory functions of 2-month-old, 10-month-old and 18-month-old female P2X7R KO mice and age-matched female C57BL/6 wildtype mice (WT mice) by buried food seeking test and olfactory avoidance test. The structure of mitochondria and synapses in olfactory bulb were observed by electron microscopy. The content of interleukin-1 (IL-1β) in olfactory bulb and transforming growth factor beta 1 (TGF-β1) in olfactory epithelium were analyzed by ELISA. The results indicated that middle and old-aged P2X7R KO mice showed better olfactory function than middle and old-aged WT mice. Mitochondrial structures were complete and more spine synapses were observed in middle and old-aged P2X7R KO mice. Compared with middle and old-aged WT mice, IL-1β content in olfactory bulb was decreased in middle and old-aged P2X7R KO mice, and there was no significant difference in TGF-β1 content in olfactory epithelium. However, worse olfactory function was observed in young-aged P2X7R KO mice compared with young-aged WT mice. Abnormal mitochondrial structure and less synapses in olfactory bulb were observed. TGF-β1 content in olfactory epithelium was significantly higher in P2X7R KO mice compared with young-aged WT mice. There was no significant difference in IL-1β content in olfactory bulb of young-aged mice. In conclusion, P2X7R knockout can improve the olfactory function of middle and old-aged mice, while it may cause damage to young-aged mice, suggesting that P2X7R plays age-specific role on olfactory functions in mice.