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LncRNA expression profile during autophagy and Malat1 function in macrophages
Zhanbing Ma1,2,3, Jing Zhang2,3, Xiangrong Xu4
1Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China.
Plos One
|August 20, 2019
Summary
Long noncoding RNAs (lncRNAs) regulate gene expression and autophagy in macrophages. The metastasis-associated lung adenocarcinoma transcript-1 (Malat1) lncRNA was found to regulate lysosomal-associated membrane protein 1 (Lamp1) expression via microRNA-23-3p.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) are critical regulators of gene expression and cellular processes like autophagy.
- Autophagy is essential for cellular homeostasis, immunity, and inflammation, particularly in macrophages.
- The specific roles and expression profiles of lncRNAs in macrophage autophagy remain largely undefined.
Purpose of the Study:
- To investigate the lncRNA expression profile during autophagy in macrophages.
- To elucidate the function of the metastasis-associated lung adenocarcinoma transcript-1 (Malat1) lncRNA in macrophage autophagy.
- To explore the regulatory mechanisms involving Malat1, microRNA-23-3p, and lysosomal-associated membrane protein 1 (Lamp1).
Main Methods:
- Transcriptomic and bioinformatics analysis to identify lncRNAs.
- Functional studies to assess the role of Malat1 in macrophages.
- Investigation of molecular interactions between Malat1, microRNA-23-3p, and Lamp1.
Main Results:
- 1112 putative lncRNAs, including 240 novel ones, were identified in macrophages during autophagy.
- 59 differentially expressed lncRNAs showed significant changes under varying conditions.
- Malat1 was found to regulate Lamp1 expression by competitively binding microRNA-23-3p, thereby releasing inhibition.
Conclusions:
- This study provides a comprehensive overview of lncRNA expression during macrophage autophagy.
- Malat1 plays a significant role in regulating autophagy in macrophages through the Malat1/microRNA-23-3p/Lamp1 axis.
- The findings offer insights into the ncRNA-mediated regulation of macrophage function and immunology, with implications for pathogenesis.
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