Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reproductive Cloning01:27

Reproductive Cloning

32.6K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.6K
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

1.3K
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
1.3K
Cardiopulmonary Resuscitation V: Advanced Airway Management Techniques01:30

Cardiopulmonary Resuscitation V: Advanced Airway Management Techniques

488
Airway management is essential in emergency and surgical medicine, ensuring ventilation and oxygenation in patients who cannot maintain their own airway. Clinicians use a range of techniques and devices to secure the airway, depending on the patient’s condition and the clinical context. Key methods include endotracheal intubation, rapid sequence intubation (RSI), supraglottic airway devices, and advanced visualization aids. In cases where these approaches fail, surgical airway...
488
What is Natural Selection?01:32

What is Natural Selection?

126.0K
Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
126.0K
Sperm Transport01:15

Sperm Transport

3.2K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
3.2K
Overview of the Reproductive System01:31

Overview of the Reproductive System

5.1K
The reproductive system generates offspring, ensuring the survival of the species. In humans, the reproductive system is complex and involves a variety of organs and hormones that work together to ensure successful reproduction.
The gonads, or primary reproductive organs, produce gametes and sex hormones. In males, the testes produce spermatozoa and testosterone, which is responsible for developing secondary male sex characteristics, including a deeper voice, larger muscles, facial and body...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced sperm selection techniques for assisted reproduction.

The Cochrane database of systematic reviews·2026
Same author

Proteomic Analysis of Cervicovaginal Fluid for Diagnostic Endometriosis Biomarker Discovery.

Proteomics. Clinical applications·2026
Same author

Endometrial receptivity-guided embryo transfer: a systematic review and meta-analysis of the evidence.

Journal of assisted reproduction and genetics·2026
Same author

Blastocyst-stage versus cleavage-stage embryo transfer in assisted reproductive technology.

The Cochrane database of systematic reviews·2026
Same author

The strength of certainty and the power of words.

Fertility and sterility·2025
Same author

Vasodilators for women undergoing fertility treatment.

The Cochrane database of systematic reviews·2025
Same journal

Interventions for smoking cessation in inpatient psychiatry settings.

The Cochrane database of systematic reviews·2026
Same journal

Mechanical thromboprophylaxis for preventing intradialytic hypotension in people undergoing maintenance haemodialysis.

The Cochrane database of systematic reviews·2026
Same journal

Prognostic models for predicting intensive care unit admission or mortality in critically ill adults not yet been admitted to the intensive care unit.

The Cochrane database of systematic reviews·2026
Same journal

Views and experiences of weight management for people living with mobility‑limiting conditions, intellectual disabilities or severe mental illness: a qualitative evidence synthesis.

The Cochrane database of systematic reviews·2026
Same journal

Non-pharmacological interventions for anxiety and depression in Parkinson's disease.

The Cochrane database of systematic reviews·2026
Same journal

Biologic drugs for induction and maintenance of remission in Crohn's disease: a network meta-analysis.

The Cochrane database of systematic reviews·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.1K

Advanced sperm selection techniques for assisted reproduction.

Sam Lepine1, Simon McDowell, Leigh M Searle

  • 1Department of Obstetrics and Gynaecology, Capital and Coast District Health Board, Wellington, New Zealand.

The Cochrane Database of Systematic Reviews
|August 20, 2019
PubMed
Summary
This summary is machine-generated.

Advanced sperm selection techniques in assisted reproductive technologies (ART) may reduce miscarriage rates but show little effect on live birth or pregnancy. Further high-quality research is needed to confirm their effectiveness in routine practice.

More Related Videos

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.7K
Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

12.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.1K
Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.7K
Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
10:07

Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract

Published on: June 6, 2019

12.9K

Area of Science:

  • Reproductive Medicine and Biology
  • Genetics and Genomics
  • Obstetrics and Gynecology

Background:

  • Assisted reproductive technologies (ART), including in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), aim to enhance fertilization and pregnancy probabilities.
  • Advanced sperm selection techniques are increasingly utilized in ART, particularly with ICSI, to identify sperm with optimal structural integrity, maturity, and DNA integrity.
  • These advanced methods encompass selection based on surface charge, apoptosis, birefringence, hyaluronic acid binding, and ultra-high magnification morphology, intended to improve ART outcomes.

Purpose of the Study:

  • To systematically evaluate the effectiveness and safety of various advanced sperm selection techniques on ART outcomes.
  • To synthesize evidence from randomized controlled trials (RCTs) comparing advanced sperm selection methods against standard ART procedures or other techniques.

Main Methods:

  • A comprehensive systematic search of multiple electronic databases and clinical trial registries was conducted up to June 2018.
  • Randomized controlled trials (RCTs) comparing advanced sperm selection techniques with standard IVF, ICSI, or alternative methods were included.
  • Primary outcomes included live birth and miscarriage rates, with secondary outcomes focusing on clinical pregnancy and adverse events. Data were analyzed using fixed-effect models, and evidence quality was assessed using GRADE methods.

Main Results:

  • Eight RCTs involving 4147 women were included, with evidence quality ranging from very low to low, limited by imprecision and bias.
  • Hyaluronic acid binding (HA-ICSI) showed potential to reduce miscarriage rates (RR 0.61, 95% CI 0.45 to 0.83) but had little to no effect on live birth or clinical pregnancy compared to standard ICSI.
  • Evidence for other techniques like Magnetic-Activated Cell Sorting (MACS) and Zeta sperm selection was very low quality, leading to uncertainty regarding their impact on live birth, miscarriage, and pregnancy rates.

Conclusions:

  • Sperm selected via hyaluronic acid binding may decrease miscarriage rates, with no significant impact on live birth or clinical pregnancy rates.
  • The effectiveness of Zeta sperm selection and other advanced techniques (e.g., MACS) remains uncertain due to very low-quality evidence.
  • Further high-quality randomized controlled trials are essential to determine the clinical utility and recommendability of these advanced sperm selection methods in routine ART practice.