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Methylated DNA Immunoprecipitation
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Genes Controlled by DNA Methylation Are Involved in Wilms Tumor Progression.

João Victor da Silva Guerra1,2, Bruna Maria de Sá Pereira3, Jéssica Gonçalves Vieira da Cruz4

  • 1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas 13083-970, Brazil.

Cells
|August 21, 2019
PubMed
Summary

DNA methylation analysis reveals distinct patterns in Wilms tumor (WT) metastasis. These findings identify potential gene signatures for understanding WT progression and metastasis formation.

Keywords:
DNA methylationDNMTTETWilms tumorcancer progressiondata integrationgene expression

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Wilms tumors (WTs) are pediatric kidney cancers.
  • Metastasis formation is a critical factor in WT prognosis.
  • Understanding the molecular mechanisms of WT metastasis is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the underlying mechanisms of metastasis formation in Wilms tumors.
  • To identify molecular differences between normal kidney, Wilms tumor, and metastatic tissues.
  • To explore potential gene signatures associated with WT metastasis.

Main Methods:

  • Comprehensive DNA methylation and gene expression analyses were performed on matched normal kidney (NK), WT blastemal component, and metastatic tissues (MT).
  • A linear Bayesian framework model was used to identify differentially methylated positions (DMPs).
  • Hierarchical clustering analysis was applied to gene expression data.

Main Results:

  • 497 DMPs were identified, discriminating NK from WT, with MT samples dividing into two distinct groups.
  • WT tissues showed hypomethylation compared to NK, while MT tissues exhibited hypermethylation.
  • Gene expression analysis revealed two distinct groups of metastases, with 44 candidate genes identified for potential metastasis signature.

Conclusions:

  • DNA methylation patterns correlate with gene expression and may control genes involved in Wilms tumor progression.
  • Distinct methylation and expression profiles exist between normal kidney, primary WT, and metastatic tissues.
  • A panel of 44 genes shows promise as a signature for predicting metastasis formation in Wilms tumors.