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Updated: Jan 20, 2026
06:54
Formulation of Zinc-Based Nanomaterials using the Eucommia ulmoides Bark Extract and their Wound Healing Potential
Published on: December 27, 2024
748
Dynamic Changes in Metabolite Accumulation and the Transcriptome during Leaf Growth and Development in Eucommia
Long Li1, Minhao Liu1, Kan Shi2
1Northwest Agriculture and Forestry University, College of Forestry, Taicheng Road No. 3, Yangling 712100, Shaanxi, China.
International Journal of Molecular Sciences
|August 21, 2019
Summary
Eucommia ulmoides leaf metabolites, including flavonoids and chlorogenic acid (CGA), were analyzed during growth. Gene expression reveals key pathways and transcription factors regulating their biosynthesis, improving genome annotation.
Area of Science:
- Plant biochemistry and molecular biology
- Metabolomics and transcriptomics
- Genomics and bioinformatics
Background:
- Eucommia ulmoides is a medicinal plant rich in bioactive compounds like chlorogenic acid (CGA) and flavonoids.
- Understanding metabolite biosynthesis and regulation during leaf development is crucial for its medicinal applications.
- Previous studies lacked comprehensive data on metabolite profiles and underlying molecular mechanisms in E. ulmoides leaves.
Purpose of the Study:
- To identify and quantify metabolites in E. ulmoides leaves during different growth stages.
- To investigate the dynamic changes in metabolite accumulation.
- To elucidate the molecular mechanisms governing metabolite biosynthesis through transcriptomic analysis.
Main Methods:
- Widely targeted metabolite profiling using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS).
- Single-molecule long-read sequencing for transcriptomic analysis.
- Co-expression network analysis to identify regulatory relationships between pathways and transcription factors.
Main Results:
- 515 analytes identified, including 127 flavonoids, 46 organic acids, and 44 amino acid derivatives.
- Flavonoid accumulation peaked in growing leaves; CGA increased steadily throughout development.
- High expression of flavonoid and phenylpropanoid pathway genes was observed in growing and old leaves, with MYB and bHLH transcription factors implicated in regulation.
Conclusions:
- Dynamic metabolic models for E. ulmoides leaf development were predicted.
- The study provides insights into the regulation of metabolite biosynthesis by transcription factors.
- Enhanced genome annotation for E. ulmoides was achieved, supporting future research.
Keywords:
Eucommia ulmoidesMYB (v-myb avian myeloblastosis viral oncogene homolog)flavonoidmetabolomeMore Related Videos
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