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Updated: Jan 20, 2026
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
Published on: August 19, 2015
A strategy to identify mixed polysaccharides through analyzing the monosaccharide composition of disaccharides
Chunyang Cao1, Lilong Wang1, Linlin Wang1
1School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
Abstract:
It is still a challenge to identify mixed polysaccharides isolated from biological tissues. The present study aimed to develop a protocol that allowed for quick characterization of mixed polysaccharides by selecting Cyclina sinensis polysaccharides as test beds. Disaccharides were gradually released by graded acid hydrolysis, separated with 3 kDa molecular weight cut-off membrane, and analyzed by HPLC-ESI-MSn. Furthermore, the monosaccharide composition of the filtrate during the gradient acid hydrolysis process and the final retentate were also determined to provide composition information of these disaccharides. Thus, 6 disaccharides were detected and identified, indicating the existence of polysaccharides constructed of corresponding repeated disaccharide fragments in C. sinensis. The present study demonstrated a potential strategy to characterize mixed polysaccharides without separation and purification.
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