Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sign Test for Matched Pairs01:17

Sign Test for Matched Pairs

385
The sign test for matched pairs offers a robust method for comparing two paired samples, often for the effects of an intervention in one of them. This method is very useful in situations where the underlying distribution of the data is unknown. The test compares two related samples—often pre- and post-treatment measurements on the same subjects—to determine if there are significant differences in their median values.
To conduct the sign test, we first calculate the differences in...
385
DNA as a Genetic Template02:05

DNA as a Genetic Template

27.4K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
27.4K
DNA as a Genetic Template02:05

DNA as a Genetic Template

9.4K
9.4K
Voltage01:13

Voltage

3.8K
The movement of electrons in a conductor requires some form of energy or work, usually provided by an external force, like a battery. This force is called the electromotive force or voltage. The voltage between two points, referred to as points "a" and "b," in an electric circuit is the energy (or work) needed to move a unit charge from point "a" to point "b," and this relationship is expressed mathematically as
3.8K
Normal Stress01:19

Normal Stress

1.4K
Normal stress is a type of stress that occurs when forces act perpendicular, or normal, to a material's cross-sectional area. This stress often arises in structures when subjected to axial loading, which is the application of force along the axis of an object. A practical example of this can be found in bridge truss members.
When a rod is under axial loading, the internal forces and corresponding stress are normal to the plane of the section, so it is termed normal stress. It's important to...
1.4K
Voltage Dividers01:14

Voltage Dividers

1.3K
In electrical circuits, resistors can be connected in series, sequentially linked one after the other. In a series configuration, the same current flows through each resistor. Ohm's law is a fundamental principle to understand the behavior of resistors in series. It expresses the voltage across these resistors in terms of the current and resistance.
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical voltage imaging: ready to spark systems neuroscience.

Current opinion in neurobiology·2026
Same author

Distinct laminar origins of sensory-evoked high-gamma and low-frequency ECoG signals revealed by optogenetics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Degraded sensory coding in a mouse model of <i>Scn2a-</i>related disorder and its rescue by CRISPRa gene activation.

bioRxiv : the preprint server for biology·2025
Same author

Distinct laminar origins of high-gamma and low-frequency ECoG signals revealed by optogenetics.

bioRxiv : the preprint server for biology·2025
Same author

The hidden cost of being uninsured: Stroke mortality after mechanical thrombectomy.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association·2025
Same author

Reward history guides focal attention in whisker somatosensory cortex.

Nature communications·2025

Related Experiment Video

Updated: Jan 20, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.4K

A normalized template matching method for improving spike detection in extracellular voltage recordings.

Keven J Laboy-Juárez1,2, Seoiyoung Ahn3, Daniel E Feldman3

  • 1Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA, 94720, USA. klaboyjuarez@g.harvard.edu.

Scientific Reports
|August 21, 2019
PubMed
Summary

A new template matching method improves spike detection in neural recordings, capturing more neuronal signals missed by traditional fixed thresholds, especially during intense brain activity.

More Related Videos

Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice
08:59

Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice

Published on: May 26, 2023

3.7K
Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
10:36

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Published on: June 13, 2017

15.5K

Related Experiment Videos

Last Updated: Jan 20, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.4K
Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice
08:59

Author Spotlight: Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice

Published on: May 26, 2023

3.7K
Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
10:36

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors

Published on: June 13, 2017

15.5K

Area of Science:

  • Neuroscience
  • Computational Neuroscience

Background:

  • Spike sorting is crucial for analyzing neural activity.
  • Standard spike detection methods using fixed thresholds struggle with high firing rates and noisy data.

Purpose of the Study:

  • To develop and evaluate a data-driven spike detection method using template matching.
  • To improve the accuracy and robustness of spike detection in extracellular recordings.

Main Methods:

  • Developed a spike detection algorithm based on sliding cosine similarity between voltage signals and mean spike waveforms.
  • Tested the method in the whisker somatosensory cortex (S1) of anesthetized mice.
  • Benchmarked performance against the standard fixed threshold method using in vivo and simulated data.

Main Results:

  • The template matching method consistently detected spikes missed by the fixed threshold, particularly for strong stimuli (40-70% increase).
  • Improved detection during dense spiking epochs and yielded sharper sensory tuning estimates.
  • Detected 85-90% of spikes compared to 70% for the fixed threshold, showing greater tolerance to noise and high firing rates.

Conclusions:

  • A simple template matching approach significantly enhances single-unit spike detection in cortical physiology.
  • This method offers a more reliable way to analyze neural activity, especially in challenging recording conditions.
  • The improved detection accuracy can lead to more precise neurophysiological measurements.