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Human Fibroblast Gene Expression Modulation Using 940 NM Diode Laser.
Rebeca Illescas-Montes1,2, Lucía Melguizo-Rodríguez1,2, Olga García-Martínez1,2
1Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.
Scientific Reports
|August 21, 2019
Summary
Low-level laser therapy (LLLT) using a 940-nm diode laser significantly impacts fibroblast gene expression. This photobiomodulation promotes cell growth and differentiation, supporting its therapeutic potential for wound repair.
Area of Science:
- Regenerative Medicine
- Biophotonics
- Cell Biology
Background:
- Low-Level Laser Therapy (LLLT) leverages photobiomodulation for regenerative purposes across various clinical fields.
- Fibroblasts are crucial cells in tissue regeneration and wound healing, responding to cellular signaling pathways.
- Understanding LLLT's effect on fibroblast gene expression is key to optimizing therapeutic outcomes.
Purpose of the Study:
- To investigate the impact of 940-nm diode laser irradiation on the gene expression of key markers in human fibroblasts.
- To analyze changes in genes related to fibroblast growth, differentiation, and migration following laser treatment.
- To assess the dose-dependent effects of LLLT on fibroblast cellular functions.
Main Methods:
- Human fibroblasts were treated with a 940-nm diode laser at single (T1) or double (T2) doses (0.5 Watts, 4 J/cm²).
- Quantitative real-time polymerase chain reaction (q-RT-PCR) was employed to measure gene expression levels.
- Expression of growth factors (FGF, CTGF, VEGF), signaling molecules (TGF-β1, TGFβRs), matrix components (MMP2, DDR2), and cytoskeletal proteins (α-actin, fibronectin, decorin, elastin) was quantified.
Main Results:
- Significant upregulation of FGF, TGF-β1, TGFβR1, TGFβR2, α-actin, fibronectin, decorin, DDR2, and MMP2 was observed post-laser treatment.
- A notable decrease in elastin expression was seen in both T1 and T2 groups, with CTGF also decreasing in T2.
- These gene expression changes indicate a biostimulatory effect, enhancing fibroblast proliferation and promoting differentiation into myofibroblasts.
Conclusions:
- 940-nm diode laser therapy effectively modulates fibroblast gene expression, promoting key pathways for tissue repair.
- The observed changes support LLLT's role in accelerating wound healing through enhanced fibroblast activity.
- LLLT demonstrates significant therapeutic potential for regenerative applications, particularly in wound management.
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