Related Experiment Video
Updated: Jan 20, 2026
11:28
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
Published on: April 28, 2015
10.8K
Application of Molecular Methods for Carbapenemase Detection
Anastasia Bilozor1,2, Arta Balode3, Giorgi Chakhunashvili4
1Department of Microbiology, Central Laboratory, East-Tallinn Central Hospital, Tallinn, Estonia.
Frontiers in Microbiology
|August 21, 2019
Summary
This study compared carbapenemase detection methods for Klebsiella pneumoniae in Europe. Whole genome sequencing and MALDI-TOF showed high sensitivity, identifying NDM and OXA-48 as common carbapenemases, crucial for antimicrobial resistance surveillance.
Area of Science:
- Microbiology
- Infectious Diseases
- Clinical Diagnostics
Background:
- Carbapenem non-susceptible Klebsiella pneumoniae poses a significant global health threat.
- Accurate and sensitive detection of carbapenemases is crucial for effective treatment and infection control.
Purpose of the Study:
- To evaluate and compare the performance of various carbapenemase detection methods.
- To assess the prevalence of different carbapenemases in K. pneumoniae isolates from Northern and Eastern Europe.
Main Methods:
- Screening of 5,001 K. pneumoniae isolates using disk diffusion, Vitek 2, or Phoenix systems.
- Confirmation of carbapenemase production using Luminex assay, whole genome sequencing, MALDI-TOF, and immunochromatography.
- Minimal inhibitory concentration determination for Meropenem.
Main Results:
- 171 (3.42%) carbapenemase screening-positive isolates were identified.
- Whole genome sequencing and MALDI-TOF assay demonstrated the highest sensitivity (>96.3%).
- NDM (54 isolates) and OXA-48 (26 isolates) were the most prevalent carbapenemases detected.
Conclusions:
- Multiple carbapenemase types are circulating in the studied European regions.
- A combination of detection methods ensures high sensitivity (>95%) for accurate carbapenemase identification.
- Continued surveillance and method optimization are recommended for precise antimicrobial resistance detection.
Keywords:
Klebsiella pneumoniaeMALDI-TOF carbapenemase assaycarbapenemasesluminex carbapenemases assaywhole genome sequencingMore Related Videos
Related Concept Videos
01:20Applications of Molecular Taxonomy
530
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
530
01:18Effects of EDTA on End-Point Detection Methods
627
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
627
03:07Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
29.7K
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws.
29.7K
02:00Molecular Models
43.6K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.6K
01:19Precipitation Titration: Endpoint Detection Methods
5.8K
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
In the Volhard method, a standard excess of AgNO3 is first added to the...
5.8K
03:51Molecular Orbital Theory II
27.0K
Molecular Orbital Energy Diagrams
27.0K