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Gene encoding a minor extracellular protease in Bacillus subtilis.

A Sloma1, A Ally, D Ally

  • 1BioTechnica International, Inc., Cambridge, Massachusetts 02140.

Journal of Bacteriology
|December 1, 1988
PubMed
Summary

Researchers identified the Bacillus subtilis extracellular protease gene (epr). This gene encodes a protein partially homologous to other B. subtilis proteases and is not essential for bacterial growth or sporulation.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Enzymology

Background:

  • Bacillus subtilis is a well-studied bacterium with various secreted enzymes.
  • Extracellular proteases play roles in nutrient acquisition and extracellular matrix remodeling.
  • Understanding the diversity and function of proteases in B. subtilis contributes to microbial biochemistry knowledge.

Purpose of the Study:

  • To identify and characterize the gene encoding a minor extracellular protease in Bacillus subtilis.
  • To elucidate the structural and functional properties of the newly identified protease.
  • To determine the gene's location and its necessity for bacterial growth and sporulation.

Main Methods:

  • Gene identification and cloning from Bacillus subtilis.
  • Bioinformatic analysis of the deduced amino acid sequence.

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  • Deletion analysis to determine functional domains.
  • Gene mapping experiments.

    • Main Results:

    • The gene, designated epr, was identified and encodes a 645-amino acid protein.
    • The Epr protein showed partial homology to subtilisin (Apr) and the major internal serine protease (ISP-1).
    • Deletion analysis revealed that the C-terminal 240 amino acids are dispensable for protease activity.
    • The C-terminal region contains unusual features: high lysine content and a repeated 44-amino acid sequence.
    • The epr gene was mapped near sacA and is not required for growth or sporulation.

    Conclusions:

    • A novel minor extracellular protease gene (epr) has been characterized in Bacillus subtilis.
    • The Epr protein possesses unique structural elements in its C-terminus.
    • The epr gene is not essential for Bacillus subtilis viability under standard laboratory conditions.