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Nucleotide sequence of the Bacillus subtilis phoR gene
T Seki1, H Yoshikawa, H Takahashi
1Institute of Applied Microbiology, University of Tokyo, Japan.
Journal of Bacteriology
|December 1, 1988
Summary
The Bacillus subtilis phoR gene sequence was determined, revealing its role in regulating alkaline phosphatase and phosphodiesterase. Its amino acid sequence shows homology to the Escherichia coli phoR gene product.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Bacillus subtilis regulates alkaline phosphatase and phosphodiesterase formation.
- The phoR gene is a key regulator in this process.
- Understanding phoR's genetic and protein sequence is crucial for deciphering its regulatory function.
Purpose of the Study:
- To determine the nucleotide sequence of the Bacillus subtilis phoR gene.
- To analyze the predicted protein sequence and its relationship to known regulatory elements.
- To investigate the homology between Bacillus subtilis PhoR and Escherichia coli PhoR.
Main Methods:
- DNA sequencing of the phoR gene in Bacillus subtilis.
- Bioinformatic analysis to predict open reading frame and amino acid sequence.
- Sequence alignment and homology comparison with Escherichia coli PhoR.
Main Results:
- The nucleotide sequence of Bacillus subtilis phoR was determined.
- An open reading frame of 1,740 base pairs (579 amino acids) was predicted.
- The deduced amino acid sequence showed significant homology to the Escherichia coli phoR gene product.
Conclusions:
- The phoR gene sequence provides insights into its regulatory function in Bacillus subtilis.
- The homology to E. coli PhoR suggests a conserved sensory role in the pho regulon.
- This study lays the groundwork for further investigation into PhoR-mediated gene regulation.